Proteomic analysis indicates that overexpression and nuclear translocation of lactoylglutathione lyase (GLO1) is associated with tumor progression in murine fibrosarcoma.

Abstract

Lactoylglutathione lyase (GLO1), a ubiquitously expressed methylglyoxal (MG) detoxification enzyme, is implicated in the progression of various human malignant diseases. However, the role of GLO1 in the development or progression of murine fibrosarcoma is still unclear. We performed proteomic analysis to identify differences in the intracellular proteins of the regressive tumor cell line QR-32 and the inflammatory cell-promoting progressive tumor cell line QRsP-11 of murine fibrosarcoma by 2DE combined with MS. Seven upregulated proteins were identified in QRsP-11 compared to QR-32 cells, namely, GLO1, annexin A1, adenylate kinase isoenzyme 1, transcription factor BTF3, myosin light polypeptide 6, low molecular weight phosphotyrosine protein phosphatase and nucleoside diphosphate kinase B. Heat shock protein beta-1 (HspB1), a methylglyoxal-adducted protein, is concomitantly over-expressed in QRsP-11 as compared to QR-32 cells. We also found out that GLO1 is translocated into the nucleus to a higher extent in QRsP-11 compared to QR-32 cells, which can be reversed by using a MEK inhibitor (U0126). Moreover, U0126 and GLO1 siRNA can inhibit cell proliferation and migration in QRsP-11 cells. Our data suggest that overexpression and nuclear translocation of GLO1 might be associated with tumor progression in murine fibrosarcoma.

• Authors: Yufeng Wang, Yasuhiro Kuramitsu, Kazuhiro Tokuda, Futoshi Okada, Byron Baron, Junko Akada, Takao Kitagawa, Kazuyuki Nakamura
• Journal: Electrophoresis (Electrophoresis) Vol. 35 Issue 15 Pg. 2195-202 (Aug 2014) ISSN: 1522-2683 [Electronic] Germany
• PMID: 24532130 (Publication Type: Journal Article)
• Copyright: © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Heat-Shock Proteins
• Hsbp1 protein, mouse
• Molecular Chaperones
• Neoplasm Proteins
• Proteome
• RNA, Small Interfering
• Lactoylglutathione Lyase

Topics

• Animals
• Cell Line, Tumor
• Cell Nucleus (metabolism)
• Cell Proliferation
• Chromatography, Liquid
• Electrophoresis, Gel, Two-Dimensional
• Fibrosarcoma (metabolism)
• Heat-Shock Proteins (metabolism)
• Lactoylglutathione Lyase (analysis, chemistry, genetics, metabolism)
• MAP Kinase Signaling System
• Mice
• Molecular Chaperones
• Neoplasm Proteins (metabolism)
• Proteome (analysis, chemistry)
• Proteomics (methods)
• RNA, Small Interfering (genetics, metabolism)
• Tandem Mass Spectrometry