Abstract | INTRODUCTION: Our preliminary study on repressing colorectal tumors by recombinant adenoviruses (Ads) delivering the human ERβ gene (Ad-ERβ) has achieved positive result. METHODS: In this study, hydrophobic fluorescent dyes ICG-Der-01 was entrapped into the N-succinyl-N'-octyl chitosan (SOC) micelles to form the near infrared absorbing dyes SOC-ICG-Der-01 and SOC-ICG-Der-01 mediated near infrared laser (SOC-ICG-Der-01/NIR) thermotherapy was combined with Ad-ERβ gene therapy to regress colon cancer in vivo. RESULTS: Firstly, the antitumor efficacies of SOC-ICG-Der-01/NIR thermotherapy were investigated on S180 ascites tumor-bearing mice. Results indicated that, the average tumor volume of SOC-ICG-Der-01/NIR group was the smallest among the three treatment groups. Then, thermotherapy with SOC-ICG-Der-01/NIR combined with Ad-ERβ gene therapy to treat HCT-116 colon cancer xenograft model was investigated. Further results demonstrated that, SOC-ICG-Der-01/NIR thermotherapy showed the significantly inhibitory efficiency compared with control group and Ad-ERβ enhanced the therapeutic effect of SOC-ICG-Der-01/NIR. CONCLUSION: These findings demonstrated that combined administration of Ad-ERβ with SOC-ICG-Der-01/NIR thermotherapy represents a promising colon cancer therapeutic strategy.
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Authors | Zhenzhen Tu, Yuxiang Ma, Walter Akers, Samuel Achilefu, Yueqing Gu |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 140
Issue 4
Pg. 623-32
(Apr 2014)
ISSN: 1432-1335 [Electronic] Germany |
PMID | 24531912
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Estrogen Receptor beta
- Fluorescent Dyes
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Topics |
- Adenoviridae
(genetics)
- Animals
- Colorectal Neoplasms
(diagnosis, genetics, therapy)
- Combined Modality Therapy
- Estrogen Receptor beta
(genetics)
- Fluorescent Dyes
- Genetic Therapy
- Genetic Vectors
(administration & dosage)
- Humans
- Hyperthermia, Induced
- Laser Therapy
- Mice
- Mice, Nude
- Sarcoma 180
(genetics, therapy)
- Tumor Cells, Cultured
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