Protection by a transdermal patch containing eserine and pralidoxime chloride for prophylaxis against (±)-Anatoxin A poisoning in rats.

Abstract

The prophylactic and neuroprotective impact of a transdermal patch containing eserine and pralidoxime chloride (2-PAM) against (±)-Anatoxin A poisoning was investigated using Wistar strain albino rats. Rats were smooth-shaved on the dorsal side, attached with a drug-in-adhesive matrix type prophylactic transdermal patch for 72 h and challenged with subcutaneous injection of three doses (1.0, 1.5 and 2.0×LD50) of (±)-Anatoxin A. The LD50 value of (±)-Anatoxin A was determined to be 1.25mg/kg, and at this particular dose (1.0×LD50) of toxin induced severe clinical symptom including extreme seizures in rats, resulting acute brain injuries in discrete brain regions, leading to 100% mortality within 5 min. The anticonvulsant effect, antiarrythmic effect, nerve conduction study, clinical observations and mortality, neuroprotective effect as well as skin histopathology of the prophylactic transdermal patch against (±)-Anatoxin A poisoning were investigated systematically. It was found that seizures, tachycardia, nerve damage, clinical symptoms, brain injuries and mortality induced by such lethal toxin were effectively prevented by the prophylactic patch treatment up to certain LD50 level. Hence, it could be a choice of potential therapeutic regimen against such lethal poisoning.

• Authors: Subham Banerjee, Pronobesh Chattopadhyay, Animesh Ghosh, Manash Pratim Pathak, Jyotchna Gogoi, Vijay Veer
• Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (Eur J Pharm Sci) Vol. 56 Pg. 28-36 (Jun 02 2014) ISSN: 1879-0720 [Electronic] Netherlands
• PMID: 24530375 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Arrhythmia Agents
• Anticonvulsants
• Cyanobacteria Toxins
• Neuroprotective Agents
• Pralidoxime Compounds
• Tropanes
• anatoxin a
• Physostigmine
• pralidoxime

Topics

• Animals
• Anti-Arrhythmia Agents (administration & dosage)
• Anticonvulsants (administration & dosage)
• Arrhythmias, Cardiac (chemically induced, drug therapy, pathology, physiopathology)
• Brain (drug effects, pathology)
• Cyanobacteria Toxins
• Male
• Neuroprotective Agents (administration & dosage)
• Physostigmine (administration & dosage)
• Pralidoxime Compounds (administration & dosage)
• Rats, Wistar
• Seizures (chemically induced, drug therapy, pathology, physiopathology)
• Skin (drug effects, pathology)
• Transdermal Patch
• Tropanes (poisoning)