Structural elucidation and in vitro antitumor activity of a novel oligosaccharide from Bombyx batryticatus.

Abstract

An oligosaccharide BBPW-2 was isolated and purified from Bombyx batryticatus, its molecular weight was 2.0 × 10(3)Da, and its structure was elucidated by compositional, methylation and NMR analysis. Our results showed that BBPW-2 consisted of β-D-(1 → 2,6)-glucopyranose and β-D-(1 → 2,6)-mannosyl units serving as the backbone, α-D-(1 → 2)-galactopyranose and α-D-(1 → 3)-mannosyl units as branches, and α-D-Manp and β-D-Glcp as terminals. The in vitro inhibitory activity of BBPW-2 was measured using MTT and and crystal violet assays, which suggested that BBPW-2 had direct cytotoxic effects on the cancer cell lines HeLa and HepG2 (particularly HeLa cells), and had a long-term antiproliferative effect on MCF-7 cells, respectively. Apoptosis and cellcycle analysis of HeLa cells showed that BBPW-2 induced cellcycle disruption in the G0/G1 and G2/M phases accompanied by an impressive increment of early apoptotic cells and late apoptotic and necrotic cells. These results suggest that BBPW-2 could be a potential chemotherapeutic drug and its antitumor effects deserve further study.

• Authors: Xue Jiang, Zuofa Zhang, Yajie Chen, Zhenteng Cui, Liangen Shi
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 103 Pg. 434-41 (Mar 15 2014) ISSN: 1879-1344 [Electronic] England
• PMID: 24528751 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014. Published by Elsevier Ltd.

Chemical References

• Antineoplastic Agents
• Oligosaccharides

Topics

• Animals
• Antineoplastic Agents (chemistry, isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Bombyx (chemistry)
• Carbohydrate Conformation
• Cell Cycle (drug effects)
• Cell Proliferation (drug effects)
• Dose-Response Relationship, Drug
• Drug Screening Assays, Antitumor
• HeLa Cells
• Hep G2 Cells
• Humans
• MCF-7 Cells
• Oligosaccharides (chemistry, isolation & purification, pharmacology)
• Structure-Activity Relationship
• Tumor Cells, Cultured