Abstract | OBJECTIVE: To investigate whether rosiglitazone has renal protective effects in diet-induced obese rats. METHODS: Wistar rats were randomly divided into normal control (NC), obesity (OB) and rosiglitazone (OB + R) group (n = 8 in each group). The NC group was fed with normal diet. The OB and OB + R groups were fed with high-fat diet. Four weeks later, rosiglitazone [4 mg/(kg x d)] was given to the OB + R group by oral gavage. The other two groups were given the same amount of physiological saline in the same manner. After 24 weeks, urinary albumin/ creatinine ratio (ACR) was measured. Endothelial function was determined by measuring vasodilatation of aorta. Renal tissues were collected for morphological and CD31 immunohistochemistry. Glomerular vascular endothelial growth factor ( VEGF) and nitric oxide (NO) levels were measured. RESULTS:
Body weight, visceral fat, plasma free fatty acids (FFAs), plasma triglyceride and ACR levels increased significantly in the obese rats (P < 0.01). Rosiglitazone intervention decreased visceral fat, plasma FFAs, plasma triglyceride and ACR levels (P< 0.01), which were still higher than NC group (P < 0.01). ACR levels of the OB group were higher than those of NC group (P < 0.01), while those of OB + R group were lower than those in OB group (P < 0.01), but still higher than those of NC group (P < 0.05). Endothelium-dependent vasodilatation was impaired in the obese rats. Rosiglitazone intervention could enhance acetylcholine-induced vasorelaxation and improved endothelium-dependent vasodilatation (P < 0.05), which was similar to that in NC group (P > 0.05). Morphological and immunohistochemistry results showed glomerulomegalia, increased glomerular CD31 expression and increased proliferation of glomerular endothelial cells, which were improved by rosiglitazone (P < 0.05). Obese rats showed increased glomerular VEGF and reduced NO levels (P < 0.05). This imbalance of VEGF/NO was partly improved by rosiglitazone intervention (P < 0.05). CONCLUSION:
Rosiglitazone reduces urinary albumin excretion and has renal protective effects by improving the imbalance of VEGF/NO and endothelial dysfunction in diet-induced obese rats.
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Authors | Xiao-Dong Sun, Ye-Rong Yu |
Journal | Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
(Sichuan Da Xue Xue Bao Yi Xue Ban)
Vol. 45
Issue 1
Pg. 24-8, 33
(Jan 2014)
ISSN: 1672-173X [Print] China |
PMID | 24527576
(Publication Type: Journal Article)
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Chemical References |
- Fatty Acids, Nonesterified
- Thiazolidinediones
- Triglycerides
- Vascular Endothelial Growth Factor A
- vascular endothelial growth factor A, rat
- Rosiglitazone
- Nitric Oxide
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Topics |
- Animals
- Body Weight
- Diet, High-Fat
- Fatty Acids, Nonesterified
(blood)
- Intra-Abdominal Fat
- Kidney Glomerulus
(drug effects)
- Nitric Oxide
(metabolism)
- Obesity
(drug therapy)
- Rats
- Rats, Wistar
- Rosiglitazone
- Thiazolidinediones
(pharmacology)
- Triglycerides
(blood)
- Vascular Endothelial Growth Factor A
(metabolism)
- Vasodilation
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