Abstract | CONTEXT: OBJECTIVE: DESIGN: This was a cross-sectional study conducted in an academic hospital. SUBJECTS: Between 2010 and 2012 a total of 404 female patients with primary osteoporosis (200 Han and 204 Uygur) and 316 healthy control subjects (160 Han and 156 Uygur) were recruited to determine the distribution of C/T single nucleotide polymorphism of the CTR gene. PCR-restriction fragment length polymorphism was used at the 1377-bp site. RESULTS: The frequency of polymorphic C/T alleles of the calcitonin receptor gene in each group fit the Hardy-Weinberg equilibrium model. There was no statistically significant difference in genotypes (P = 0.922) or allele frequency (P = 0.654) between the Xinjiang Han postmenopausal osteoporosis patients and the controls. Similarly, there was no difference in genotypes (P = 0.897) or allele frequency (P = 0.825) between Xinjiang Uygur postmenopausal osteoporosis patients and the controls. Moreover, there was no significant difference (P = 0.86) between the combination of both ethnic groups and controls. In contrast, compared to these two ethnic groups, Han CC type accounted for 67.8%, CT 30.0%, and TT 2.2%, whereas Uighur CC type accounted for 55.6%, CT 33.3%, and TT 11.2%, which is statistically significant between Han and Uygur CTR genotypes (P = 0.006). Allele frequency of C accounted for 82.8% and T for 17.2% in Han, whereas C accounted for 72.2% and T for 27.8% in Uygur (P = 0.001). CONCLUSION: There was no statistically significant difference in CTR gene nucleotide sequence polymorphisms at 1377-bp between Chinese Xinjiang Han and Uygur patients with primary osteoporosis, suggesting that this CTR gene polymorphism may not affect incidence of osteoporosis. However, there was a significant difference in CTR gene nucleotide sequence polymorphism at the 1377-bp site between Chinese Xinjiang Han and Uygur, but the importance of this difference needs further study.
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Authors | J Xu, Y Gao, J Yin, X Zhao, H Wang, H Yuan, F Wang |
Journal | The journal of nutrition, health & aging
(J Nutr Health Aging)
Vol. 18
Issue 2
Pg. 204-8
( 2014)
ISSN: 1760-4788 [Electronic] France |
PMID | 24522475
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Aged
- Alleles
- Amino Acid Sequence
- Asian People
- Cross-Sectional Studies
- Ethnicity
- Female
- Gene Frequency
- Genetic Predisposition to Disease
- Genotype
- Humans
- Incidence
- Middle Aged
- Molecular Sequence Data
- Osteoporosis
(ethnology, genetics)
- Polymorphism, Single Nucleotide
- Postmenopause
- Receptors, Calcitonin
(genetics, metabolism)
- Sequence Analysis, DNA
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