Inhibition of hepatitis B virus gene expression & replication by crude destruxins from Metarhizium anisopliae var. dcjhyium.

Abstract

BACKGROUND & OBJECTIVES:

Destruxin A, destruxin B and destruxin E isolated from entomopathogenic fungus Metarhizium anisopliae showed a strong suppressive effect on the replication of hepatitis B virus (HBV) in human hepatoma cells. In this study, the anti-HBV effects of the crude destruxins extracted from M. anisopliae var. dcjhyium were detected both in vitro and in vivo.

METHODS:

HepG2.2.15 cells were cultured to observe the inhibitory effects of the crude destruxins on the gene expression and replication of HBV by radioimmunoassay detection and real-time quantitative PCR. In vivo, duck HBV (DHBV)-infected ducks were treated with the crude destruxins at 2.0, 4.0, 6.0 μg/kg once a day for 15 days, DHBV DNA was examined by real-time quantitative PCR.

RESULTS:

The crude destruxins suppressed the replication of HBV-DNA and the production of HBsAg and HBeAg with IC 50 of about 1.2 and 1.4 μg/ml. Transcript of viral mRNA was significantly suppressed by the crude destruxins in HepG2.2.15 cells. In vivo, the duck serum DHBV-DNA levels were markedly reduced in the group of the crude destruxins.

INTERPRETATION & CONCLUSIONS:

The crude destruxins inhibited the gene expression and replication of HBV both in vitro and in vivo, and their anti-HBV effect was stronger than that with destruxin B. Our results indicate that the crude destruxins from M.anisopliae var. dcjhyium may be potential antivirus agents. Further studies need to be done to confirm these findings.

• Authors: Cong Dong, Jiuru Yu, Ying Zhu, Changjin Dong
• Journal: The Indian journal of medical research (Indian J Med Res) Vol. 138 Issue 6 Pg. 969-76 (Dec 2013) ISSN: 0971-5916 [Print] India
• PMID: 24521644 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Viral
• Depsipeptides
• Fungal Proteins
• Plant Extracts
• destruxin A
• destruxin B
• destruxin E

Topics

• Animals
• DNA, Viral (drug effects)
• Depsipeptides (administration & dosage, isolation & purification)
• Ducks (virology)
• Fungal Proteins (administration & dosage, isolation & purification)
• Gene Expression Regulation, Viral (drug effects)
• Hep G2 Cells
• Hepatitis B (drug therapy, virology)
• Hepatitis B Virus, Duck (drug effects)
• Hepatitis B virus (drug effects, genetics)
• Humans
• Metarhizium
• Plant Extracts (chemistry)
• Virus Replication