[Rapid diagnosis of experimental tumor by FITC-Con A lectin--a comparative study of smear and section specimens].

Abstract

FITC-Con A staining as a rapid diagnostic method for tumor cells was applied to the tumors smeared on glass slide and section specimens to evaluate the reactivity with FITC-Con A. Good staining results were obtained in smear specimens with clear fluorescence on the membrane of tumor cells. Con A and LCH lectins bound well with tumor cells to produce strong fluorescence in comparison with PEA and DBA. It indicates that tumor cells expressed dominantly the receptors of alpha-D-glucose and alpha-D-mannose sugar chain on the membrane of tumor cells. From these results it was concluded that FITC-Con A staining method applied to smear specimens is more advantageous in the rapidity and the simplicity for tumor cell diagnosis than section specimen method.

• Authors: J Sasaki, D Sawamura, M Kitagawa, C R Ahsan, C Lü, J Masumori
• Journal: Gan to kagaku ryoho. Cancer & chemotherapy (Gan To Kagaku Ryoho) Vol. 15 Issue 4 Pt 1 Pg. 649-53 (Apr 1988) ISSN: 0385-0684 [Print] Japan
• PMID: 2451471 (Publication Type: Comparative Study, English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fluoresceins
• fluorescein isothiocyanate-concanavalin A
• Concanavalin A
• Fluorescein-5-isothiocyanate

Topics

• Animals
• Colonic Neoplasms (pathology)
• Concanavalin A (analogs & derivatives)
• Fluorescein-5-isothiocyanate (analogs & derivatives)
• Fluoresceins
• Mice
• Mice, Inbred BALB C
• Microtomy
• Specimen Handling (methods)
• Staining and Labeling (methods)
• Tumor Cells, Cultured