The effect of 5'-amino-5'-deoxythymidine on the pattern of polyadenylation of herpes simplex virus type 1 ribonucleic acid.

Abstract

Treatment of herpes simplex virus type 1 (HSV-1) infected Vero cells with 5'-amino-5'-deoxythymidine (AdThd) causes a 65% reduction in the amount of total viral RNA present at late times of infection. This decrease is apparent as early as 6 hours post infection, affecting the levels of viral cytoplasmic polyadenylated (poly(A+] species to a greater extent than non-polyadenylated (poly(A-] RNA. Cytoplasmic viral poly(A+) RNA is present in AdThd-treated cultures at only 10% control poly(A+) levels, yet there is no evidence of a direct inhibition of RNA polyadenylation. Underrepresentation of RNA species in the cytoplasm is not sequence-specific. The results suggest that the antiviral activity of AdThd may involve not only incorporation into viral DNA, with a resultant decrease in viral transcription, but also perturbation of the relative amounts of viral poly(A+) RNA and poly(A-) RNA.

• Authors: M L Zucker, S Dube, W H Prusoff
• Journal: Virus research (Virus Res) Vol. 9 Issue 2-3 Pg. 221-32 (Feb 1988) ISSN: 0168-1702 [Print] Netherlands
• PMID: 2451365 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Dideoxynucleosides
• RNA, Messenger
• RNA, Viral
• Poly A
• 5'-amino-5'-deoxythymidine
• RNA
• Thymidine

Topics

• Animals
• Cell Nucleus (metabolism)
• Cytoplasm (metabolism)
• Dideoxynucleosides
• Poly A (metabolism)
• RNA (metabolism)
• RNA Processing, Post-Transcriptional (drug effects)
• RNA, Messenger
• RNA, Viral (metabolism)
• Simplexvirus (drug effects, metabolism)
• Thymidine (analogs & derivatives, pharmacology)
• Vero Cells