Abstract | CONTEXT: OBJECTIVES: EXPERIMENTAL DESIGN: We evaluated the phenotypic effects of DZNep in thyroid cancer cells and examined the effects of DZNep alone or in combination with PRIMA-1 on cell proliferation, the cell cycle, apoptosis, and xenograft tumor growth. RESULTS:
DZNep induced enhancer of zeste homolog 2 depletion and trimethylated lysine 27 in H3 histone (H3K27me3) mark reduction in all thyroid cancer cells; however, only TP53 wild-type cells exhibited growth inhibition with DZNep treatment. In these cells, DZNep caused p53 protein accumulation through up-regulation of USP10 expression, resulting in activation of the p53 pathway, contributing to inhibition of cell growth. Conversely, TP53 mutant-type cells were resistant to DZNep. Strikingly, the combination of DZNep with PRIMA-1 restored the sensitivity of TP53 mutant-type cells to DZNep. A similar antitumor effect of DZNep and PRIMA-1 alone or in combination was also seen in xenograft tumor models. CONCLUSION: Our data demonstrated that DZNep responsiveness was strongly associated with TP53 genomic status in thyroid cancer cells. Reactivation of p53 restored the sensitivity of TP53 mutant-type cells to DZNep. Thus, a combined therapeutic strategy may be effective in treating thyroid cancer cells (or patients) harboring mutant p53.
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Authors | Bo Cui, Qi Yang, Haixia Guan, Bingyin Shi, Peng Hou, Meiju Ji |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 99
Issue 6
Pg. E962-70
(Jun 2014)
ISSN: 1945-7197 [Electronic] United States |
PMID | 24512488
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Aza Compounds
- Bridged Bicyclo Compounds, Heterocyclic
- Mutant Proteins
- TP53 protein, human
- Tumor Suppressor Protein p53
- 3-deazaneplanocin
- 2,2-bis(hydroxymethyl)-1-azabicyclo(2,2,2,)octan-3-one
- Adenosine
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Topics |
- Adenosine
(administration & dosage, analogs & derivatives, pharmacology)
- Animals
- Apoptosis
(drug effects)
- Aza Compounds
(administration & dosage, pharmacology)
- Bridged Bicyclo Compounds, Heterocyclic
(administration & dosage, pharmacology)
- Cell Cycle
(drug effects)
- Cell Line, Tumor
- Drug Resistance, Neoplasm
(drug effects, genetics)
- Drug Synergism
- Humans
- Mice
- Mice, Nude
- Mutant Proteins
(drug effects)
- Thyroid Neoplasms
(drug therapy, pathology)
- Tumor Suppressor Protein p53
(drug effects, genetics)
- Xenograft Model Antitumor Assays
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