Knocking down the expression of adenylate cyclase-associated protein 1 inhibits the proliferation and migration of breast cancer cells.

Abstract

Adenylate cyclase-associated protein 1 (CAP1) is a conserved protein that was found to be up-regulated in breast cancer and related to the migration of breast cancer. We verified its roles in breast cancer specimens and cell lines. In our results, 71 of 100 specimens of breast cancer showed high levels of CAP1 by immunohistochemistry. Associated with statistical analysis, we saw that CAP1 was related to the grade of breast cancer. In MDA-MB-231, the expression of CAP1 was the highest and by knocking down the expression of CAP1 in MDA-MB-231, its ability for proliferating and migrating apparently decreased and induced changes in morphology, which were related to the arrangement of F-actin. Therefore, CAP1 might be a potential molecular targeted therapy for surgery and immune treatment.

• Authors: Xia-Fei Yu, Qi-Chao Ni, Jin-Peng Chen, Jun-Fei Xu, Ying Jiang, Shu-Yun Yang, Jing Ma, Xiao-Ling Gu, Hua Wang, Ying-Ying Wang
• Journal: Experimental and molecular pathology (Exp Mol Pathol) Vol. 96 Issue 2 Pg. 188-94 (Apr 2014) ISSN: 1096-0945 [Electronic] Netherlands
• PMID: 24509166 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• CAP1 protein, human
• Cell Cycle Proteins
• Cytoskeletal Proteins

Topics

• Breast Neoplasms (genetics)
• Cell Cycle Proteins (biosynthesis, genetics)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation
• Cytoskeletal Proteins (biosynthesis, genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Gene Knockdown Techniques
• Humans
• Molecular Targeted Therapy