Abstract | BACKGROUND: METHODS: We developed an ELISA and immunoprecipitation method by using recombinant EJ protein to detect the anti-EJ of 453 patients with various autoimmune connective tissue diseases (ACTDs). We also studied the influence of 3 cytokines-IL-1β, IFN-γ and IFN-α-on the level of EJ mRNA and protein expressed by human fetal lung fibroblasts. RESULTS: CONCLUSION: We developed an ELISA to detect anti-EJ by using recombinant protein. This easy-to-use ELISA could help clarify the clinical significance of anti-EJ in ACTDs.
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Authors | Hiroaki Hane, Yoshinao Muro, Kanako Watanabe, Yasushi Ogawa, Kazumitsu Sugiura, Masashi Akiyama |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 431
Pg. 9-14
(Apr 20 2014)
ISSN: 1873-3492 [Electronic] Netherlands |
PMID | 24508626
(Publication Type: Journal Article)
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Copyright | Copyright © 2014 Elsevier B.V. All rights reserved. |
Chemical References |
- Autoantibodies
- Biomarkers
- Cytokines
- Recombinant Proteins
- Glycine-tRNA Ligase
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Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Autoantibodies
(analysis)
- Biomarkers
(blood)
- Cells, Cultured
- Child
- Child, Preschool
- Cytokines
(blood)
- Dermatomyositis
(blood, diagnosis)
- Enzyme-Linked Immunosorbent Assay
- Female
- Glycine-tRNA Ligase
(immunology)
- Humans
- Immunoprecipitation
- Lung Diseases, Interstitial
(blood, diagnosis)
- Male
- Middle Aged
- Polymyositis
(blood, diagnosis)
- Recombinant Proteins
(immunology)
- Young Adult
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