Effect of RSCs combined with COP-1 on optic nerve damage in glaucoma rat model.

Abstract

OBJECTIVE:

To explore effect of retinal stem cells (RSCs) combined with copolymer-1 (COP-1) immunotherapy on optic nerve damage in glaucoma rat model.

METHODS:

A total of 40 SD rats were selected for glaucoma model and were randomly divided into 4 groups to observe protective effects of RSCs transplantation combined with COP-1.

RESULTS:

Brain-derived neurotrophic factor (BDNF) and insulin like growth factor-1 (IGF-1) were either positive in retina of RSCs transplanted or COP-1 immunological treated rat. Positive rate of BDNF and IGF-1 and expression of mRNA and protein were significantly higher in RSCs transplantation combined with COP-1 immunotherapy treated rats compared with the other 3 groups, in which amount of apoptotic RGCs was lowest.

CONCLUSIONS:

RSCs transplantation combined with COP-1 immunotherapy can promote the secretion of BDNF and IGF-1. They protect RGCs in glaucoma rats in coordination, significantly reduce the number of apoptosis RGCs so as to alleviate the optic nerve damage. It ponits a new research direction for treatment of glaucoma.

• Authors: Wei-Cai Fu, Yan Jiang, Lin Zhang
• Journal: Asian Pacific journal of tropical medicine (Asian Pac J Trop Med) Vol. 7 Issue 4 Pg. 317-20 (Apr 2014) ISSN: 2352-4146 [Electronic] India
• PMID: 24507684 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

Chemical References

• Brain-Derived Neurotrophic Factor
• Peptides
• RNA, Messenger
• Glatiramer Acetate
• Insulin-Like Growth Factor I

Topics

• Animals
• Apoptosis
• Brain-Derived Neurotrophic Factor (metabolism)
• Cells, Cultured
• Chi-Square Distribution
• Disease Models, Animal
• Female
• Glatiramer Acetate
• Glaucoma (immunology, surgery, therapy)
• Insulin-Like Growth Factor I (metabolism)
• Male
• Microscopy, Fluorescence
• Optic Nerve Injuries (immunology, surgery, therapy)
• Peptides (pharmacology)
• RNA, Messenger (metabolism)
• Rats
• Rats, Sprague-Dawley
• Retina (chemistry, cytology)
• Stem Cell Transplantation (methods)
• Stem Cells (cytology)