Abstract | OBJECTIVE: METHODS: Both enzymes were cloned and expressed as recombinant proteins. The protein- protein interaction in the enzyme complex was identified using bifunctional chemical cross-linker, liquid chromatography-mass spectrometric analysis and homology modeling. RESULTS: The unique insertions of low complexity region at the α 2 and α 5 helices of the parasite OMPDC, characterized by single amino acid repeat sequence which was not found in homologous proteins from other organisms, was located on the OPRT-OMPDC interface. The structural models for the protein- protein interaction of the heterotetrameric (OPRT)2(OMPDC)2 multienzyme complex were proposed. CONCLUSIONS: Based on the proteomic data and structural modeling, it is surmised that the human malaria parasite low complexity region is responsible for the OPRT-OMPDC interaction. The structural complex of the parasite enzymes, thus, represents an efficient functional kinetic advantage, which in line with co-localization principles of evolutional origin, and allosteric control in protein- protein-interactions.
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Authors | Waranya Imprasittichail, Sittiruk Roytrakul, Sudaratana R Krungkrai, Jerapan Krungkrail |
Journal | Asian Pacific journal of tropical medicine
(Asian Pac J Trop Med)
Vol. 7
Issue 3
Pg. 184-92
(Mar 2014)
ISSN: 2352-4146 [Electronic] India |
PMID | 24507637
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Hainan Medical College. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Protozoan Proteins
- Recombinant Proteins
- Orotate Phosphoribosyltransferase
- Orotidine-5'-Phosphate Decarboxylase
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Topics |
- Amino Acid Sequence
- Humans
- Malaria, Falciparum
(parasitology)
- Models, Molecular
- Molecular Sequence Data
- Orotate Phosphoribosyltransferase
(chemistry, genetics, metabolism)
- Orotidine-5'-Phosphate Decarboxylase
(chemistry, genetics, metabolism)
- Plasmodium falciparum
(enzymology, genetics)
- Protein Binding
- Protein Interaction Domains and Motifs
- Protozoan Proteins
(chemistry, genetics, metabolism)
- Recombinant Proteins
(chemistry, genetics, metabolism)
- Sequence Alignment
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