Abstract | BACKGROUND AND OBJECTIVE: It is widely accepted that perforin regulatory elements are hypomethylated in CD4+ T cells from patients with active lupus, but whether this is the case in autoimmune emphysema is not known. METHODS: RESULTS: The levels of MMP-9 in BALF were higher in emphysema group than in control group (P < 0.05). The mean linear intercept and destructive index were higher in emphysema group than in control group (P < 0.05). The mean perforin gene promotor methylation of emphysema group was significantly decreased as compared with control group, while the expression levels of perforin gene were relatively higher (P < 0.05). There were more terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling-positive cells in the alveolar septa in control group than in emphysema group. CONCLUSIONS: Hypomethylation of perforin regulatory elements in CD4+ T cells may result in the lung septal cell apoptosis associated with the development of experimental autoimmune emphysema. MMP-9 may play an important role in the pathogenesis of this kind of disease.
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Authors | Cheng Zhang, Mu-Yun Yan, Ping Lu, Ping Chen, Min Yang, Xian-Wei Ye, Mei Xu, Zhang-Hong Ou Yang, Xiang-Yan Zhang |
Journal | Respirology (Carlton, Vic.)
(Respirology)
Vol. 19
Issue 3
Pg. 376-81
(Apr 2014)
ISSN: 1440-1843 [Electronic] Australia |
PMID | 24506670
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 The Authors. Respirology © 2014 Asian Pacific Society of Respirology. |
Chemical References |
- Interleukin-8
- Pore Forming Cytotoxic Proteins
- Tumor Necrosis Factor-alpha
- perforin, rat
- Matrix Metalloproteinase 9
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Topics |
- Animals
- Autoimmune Diseases
(genetics, metabolism)
- Bronchoalveolar Lavage Fluid
- CD4-Positive T-Lymphocytes
(metabolism)
- Cell Culture Techniques
- DNA Methylation
- Disease Models, Animal
- In Situ Nick-End Labeling
- Interleukin-8
(metabolism)
- Male
- Matrix Metalloproteinase 9
(metabolism)
- Pore Forming Cytotoxic Proteins
(genetics, metabolism)
- Pulmonary Emphysema
(genetics, metabolism, pathology)
- Rats
- Rats, Sprague-Dawley
- Real-Time Polymerase Chain Reaction
- Regulatory Elements, Transcriptional
(genetics)
- Sequence Analysis, DNA
- Spleen
(cytology, metabolism)
- Tumor Necrosis Factor-alpha
(metabolism)
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