Abstract | OBJECTIVE: METHODS: For initiation of PGIA, BALB/c mice received 2 intraperitoneal injections of human PG in a synthetic adjuvant on days 0 and 21. Five weeks after the first immunization, the mice were exposed to total body irradiation (7.5 Gy) and received (un)manipulated bone marrow (BM) grafts from mice with PGIA. Clinical scores, T cell reconstitution, ( antigen-specific) T cell cytokine production, and intracellular cytokine expression were determined following autologous BM transplantation (ABMT). RESULTS: ABMT resulted in amelioration and stabilization of arthritis scores. BM grafts containing T cells and T cell-depleted grafts provided the same clinical benefit, with similar reductions in PG-induced T cell proliferation and the number of PG-specific autoantibodies. In vivo reexposure to PG did not exacerbate disease. Following ABMT, basal levels of disease-associated proinflammatory cytokines ( interferon-γ [IFNγ], interleukin-17 [IL-17], and tumor necrosis factor α [TNFα]) were reduced. In addition, restimulation of T cells with PG induced a strong reduction in disease-associated proinflammatory cytokine production. Finally, although the remaining host T cells displayed a proinflammatory phenotype following ABMT, IFNγ, IL-17, and TNFα production by the newly reconstituted donor-derived T cells was significantly lower. CONCLUSION: Taken together, our data suggest that ABMT restores immune tolerance by renewal and modulation of the Teff cell compartment, leading to a strong reduction in proinflammatory ( self antigen-specific) T cell cytokine production.
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Authors | Eveline M Delemarre, Sarah T A Roord, Theo van den Broek, Evelien Zonneveld-Huijssoon, Wilco de Jager, Henk Rozemuller, Anton C Martens, Femke Broere, Nico M Wulffraat, Tibor T Glant, Berent J Prakken, Femke van Wijk |
Journal | Arthritis & rheumatology (Hoboken, N.J.)
(Arthritis Rheumatol)
Vol. 66
Issue 2
Pg. 350-6
(Feb 2014)
ISSN: 2326-5205 [Electronic] United States |
PMID | 24504807
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 by the American College of Rheumatology. |
Chemical References |
- Interleukin-17
- Proteoglycans
- Tumor Necrosis Factor-alpha
- Interferon-gamma
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Topics |
- Animals
- Arthritis, Experimental
(chemically induced, immunology, therapy)
- Autografts
- Bone Marrow Transplantation
- Disease Models, Animal
- Female
- Immune Tolerance
(physiology)
- Interferon-gamma
(metabolism)
- Interleukin-17
(metabolism)
- Mice
- Mice, Inbred BALB C
- Proteoglycans
(adverse effects)
- Stem Cell Transplantation
- T-Lymphocyte Subsets
(metabolism, pathology)
- T-Lymphocytes
(metabolism, pathology)
- T-Lymphocytes, Regulatory
(metabolism, pathology)
- Tumor Necrosis Factor-alpha
(metabolism)
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