[Protective effect of succinic acid on primary cardiomyocyte hypoxia/reoxygenation injury].

Abstract

To establish cardiomyocyte hypoxia/reoxygenation injury model by culturing primary cardiomyocytes from suckling SD rats, in order to study the effect of succinic acid on LDH leakage rate cardiomyocyte ischemia/reperfusion injury. Furthermore, flow cytometry and western blot were conducted to detect the effect of succinic acid on cardiomyocyte apoptosis, cleaved caspase-3 and p-Akt, and discuss the protective effect of succinic acid on primary cardiomyocyte hypoxia/reoxygenation injury of primary cardiomyocytes from neonatal SD rats. According to the findings of the study, succinic acid at the concentrations ranging between 31.25 mg x L(-1) and 500 mg x L(-1) had no significant effect on primary cardiomyocyte activity, and succinic acid at the concentrations of 400, 200, 100, 50 mg x L(-1) could notably reduce cardiomyocyte ischemia/reperfusion LDH leakage rate (P < 0.01 or P < 0.05, respectively). Succinic acid at the concentrations of 400 mg x L(-1) and 200 mg x L(-1) could significantly reduce the percentage of cardiomyocyte apoptosis (P < 0.05), and inhibit the protein expression of cleaved caspase-3 caused by cardiomyocyte ischemia/reperfusion (P < 0.05). Succinic acid at the concentration of 400 mg x L(-1) could remarkably increase the protein expression of cardiomyocyte Akt (P < 0.05), while succinic acid at the concentration of 200 mg x L(-1) had no obvious effect on the protein expression of cardiomyocyte Akt. Therefore, this study demonstrated that succinic acid could inhibit necrosis and apoptosis caused by cardiomyocyte hypoxia/reoxygenation by activating Akt phosphorylation.

• Authors: Xi-Lan Tang, Jian-Xun Liu, Peng Li, Wei Dong, Lei Li, Yong-Qiu Zheng, Jin-Cai Hou
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 38 Issue 21 Pg. 3742-6 (Nov 2013) ISSN: 1001-5302 [Print] China
• PMID: 24494565 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Protective Agents
• Succinic Acid
• Proto-Oncogene Proteins c-akt
• Caspase 3
• Oxygen

Topics

• Animals
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cell Hypoxia (drug effects)
• Cell Survival (drug effects)
• Cells, Cultured
• Female
• Humans
• Hypoxia (metabolism, physiopathology, prevention & control)
• Male
• Myocardial Reperfusion Injury (metabolism, physiopathology, prevention & control)
• Myocytes, Cardiac (cytology, drug effects, metabolism)
• Oxygen (metabolism)
• Protective Agents (pharmacology)
• Proto-Oncogene Proteins c-akt (metabolism)
• Rats
• Rats, Sprague-Dawley
• Succinic Acid (pharmacology)