Protective effect of montelukast, a cysteinyl leukotriene receptor-1 antagonist, against intestinal ischemia-reperfusion injury in the rat.

Abstract

OBJECTIVE:

Ischemia-reperfusion (I-R) injury of the intestine is a significant problem because the initial damage caused by ischemia is exacerbated by reperfusion. In this study, we examined the protective effect of montelukast against I-R-induced intestinal tissue damage.

MATERIALS AND METHODS:

Eight-week-old male Sprague-Dawley rats were randomly divided into three treatment groups : a sham-operated group, a group receiving I-R, and a group receiving I-R plus montelukast (I-R/M). Tissue samples were evaluated and scored histologically. The blood levels of malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH), and cardiotrophin-1 (CT-1) were measured.

RESULTS:

In the I-R group, the histological score and the levels of serum MDA and MPO were increased compared with those in the control group. In the I-R/M group, the histological score and serum MDA and MPO levels were significantly decreased compared with those in the I-R group. Additionally, compared with the IR group, the I-R/M group had increased serum GSH and CT-1 levels and a decreased intestinal injury score. Ileal sections from the I-R/M group showed minimal alterations, characterized by moderate lifting of the epithelial layer from the lamina propria, and few apoptotic enterocytes were observed compare with the number in the I-R group.

CONCLUSION:

The findings of the present study demonstrated that montelukast can protect I-R-induced intestinal damage in rats.

• Authors: A Duran, H Otiük, E H Terzi, M Tosun, H Oziiü, T Ocak, A Kiiüer
• Journal: Acta chirurgica Belgica (Acta Chir Belg) 2013 Nov-Dec Vol. 113 Issue 6 Pg. 401-5 ISSN: 0001-5458 [Print] England
• PMID: 24494466 (Publication Type: Journal Article)

Chemical References

• Acetates
• Cyclopropanes
• Cytokines
• Leukotriene Antagonists
• Quinolines
• Receptors, Leukotriene
• Sulfides
• Malondialdehyde
• cardiotrophin 1
• Peroxidase
• Glutathione
• leukotriene D4 receptor
• montelukast

Topics

• Acetates (therapeutic use)
• Animals
• Cyclopropanes
• Cytokines (blood)
• Glutathione (blood)
• Intestines (blood supply)
• Leukotriene Antagonists (therapeutic use)
• Male
• Malondialdehyde (blood)
• Mesentery (blood supply)
• Oxidative Stress (physiology)
• Peroxidase (blood)
• Quinolines (therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Receptors, Leukotriene (drug effects)
• Reperfusion Injury (prevention & control)
• Sulfides