Association between anti-U1 ribonucleoprotein antibodies and inflammatory mediators in cerebrospinal fluid of patients with neuropsychiatric systemic lupus erythematosus.

Abstract

OBJECTIVE:

We investigated possible associations between neurotoxic inflammatory mediators (IMs) and anti-U1RNP antibodies (Abs) in cerebrospinal fluid (CSF) of neuropsychiatric systemic lupus erythematosus (NPSLE).

METHODS:

Serum and CSF anti-U1RNP Abs were detected using an RNA-immunoprecipitation assay and CSF anti-U1RNP Ab levels were measured by ELISA. IFN-α, MCP-1 and IL-8 levels in CSF were determined by quantitative multiplex cytokine analysis. IM levels were compared among anti-U1RNP-positive and anti-U1RNP-negative NPSLE as well as other rheumatic disease controls (controls).

RESULTS:

Anti-U1RNP Abs were detected in serum (58%) and in CSF (18%) of 82 NPSLE patients. CSF MCP-1 levels were higher in NPSLE than in controls. CSF IFN-α level was higher in CSF anti-U1RNP Ab-positive than in -negative patients or controls. When limited to serum anti-U1RNP Ab-positive patients, however, levels of all three IMs in CSF were higher in CSF anti-U1RNP Ab-positive than in -negative patients. Anti-U1RNP Ab levels in CSF correlated with CSF MCP-1, but not IFN-α and IL-8 levels.

CONCLUSIONS:

CSF anti-U1RNP Ab positivity is associated with increased level of CSF IFN-α. MCP-1 levels correlated with CSF anti-U1RNP Ab levels, whereas the increased CSF MCP-1 was not specific to CSF anti-U1RNP Ab-positive NPSLE.

• Authors: T Yokoyama, T Fujii, S Kondo-Ishikawa, N Yamakawa, M Nakano, N Yukawa, H Yoshifuji, K Ohmura, T Mimori
• Journal: Lupus (Lupus) Vol. 23 Issue 7 Pg. 635-42 (Jun 2014) ISSN: 1477-0962 [Electronic] England
• PMID: 24493282 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

Chemical References

• Autoantibodies
• Ribonucleoprotein, U1 Small Nuclear

Topics

• Adolescent
• Adult
• Aged
• Autoantibodies (blood, cerebrospinal fluid)
• Child
• Female
• Humans
• Inflammation (blood, cerebrospinal fluid)
• Lupus Vasculitis, Central Nervous System (blood, cerebrospinal fluid, immunology)
• Male
• Middle Aged
• Ribonucleoprotein, U1 Small Nuclear (blood, cerebrospinal fluid)
• Young Adult