HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Identification and validation of PROM1 and CRTC2 mutations in lung cancer patients.

AbstractBACKGROUND:
Genetic alterations could be responsible lung cancer, the leading cause of worldwide cancer death.
METHODS:
This study investigated gene mutations in a Han Chinese family of lung cancer using the whole genome exome sequencing and subsequent Sanger sequencing validation and then confirmed alteration of prominin 1(PROM1) and cyclic AMP-response element binding protein-regulated transcription co-activator2 (CRTC2) in blood samples of 343 sporadic lung cancer patients vs. 280 healthy controls as well as in 200 pairs of lung cancer and the corresponding normal tissues using PCR-restriction fragment length polymorphism and directed DNA sequencing of PCR products.
RESULTS:
The data showed PROM1 (p. S281R) and CRTC2 (p. R379C) mutations, in 5 and 2 cases of these 343 sporadic lung cancer patients, respectively. Notably, these mutations were absent in the healthy controls. Furthermore, in the 200 lung cancer and the matched normal tissues, PROM1 mutation occurred in 3 patients (i.e., one squamous cell carcinoma and two adenocarcinomas) with a mutation frequency of 1.5%, while CRTC2 mutation occurred in 5 patients (two squamous cell carcinomas and three adenocarcinomas) with a mutation frequency of 2.5%.
CONCLUSIONS:
The data from the current study demonstrated novel PROM1 and CRTC2 mutations, which could promote lung cancer development.
AuthorsYanqi He, Yalun Li, Zhixin Qiu, Bin Zhou, Shaoqin Shi, Kui Zhang, Yangkun Luo, Qian Huang, Weimin Li
JournalMolecular cancer (Mol Cancer) Vol. 13 Pg. 19 (Jan 31 2014) ISSN: 1476-4598 [Electronic] England
PMID24484648 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • AC133 Antigen
  • Antigens, CD
  • CRTC2 protein, human
  • Glycoproteins
  • PROM1 protein, human
  • Peptides
  • Transcription Factors
Topics
  • AC133 Antigen
  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, CD (genetics)
  • Base Sequence
  • Carcinoma, Squamous Cell (genetics, pathology)
  • Exome (genetics)
  • Female
  • Genome, Human
  • Glycoproteins (genetics)
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Lung Neoplasms (genetics)
  • Male
  • Middle Aged
  • Mutation
  • Peptides (genetics)
  • Transcription Factors (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: