Abstract | OBJECTIVES: METHODS: Five pairs of RNA interference sequences were screened and ranked according to their highest inhibition rates in HEK293 cells. The plasmid with the highest inhibition rate was transfected by US into the rat myocardium after mixing with lipid MB. Twelve and 16 weeks after MI, cardiac function was measured by echocardiography, and glucose transporter-4 (GLUT-4) and high-energy phosphate levels were monitored in the myocardium before and after transfection. RESULTS: Ejection fraction (EF) decreased by 16% in the control MI group, while it decreased by 8% in the MCD inhibition group that utilized the US-mediated MB approach. Concomitant with the improved EF, high-energy phosphates were increased and lactic acid was decreased in the left ventricle (LV), with no changes in triglyceride or GLUT-4 levels. CONCLUSIONS: Inhibiting MCD by an US-mediated injection of miRNA into the rat myocardium increased energy reserves in the LV after MI, most likely by limiting lactic acidosis and improving cardiac function without increasing lipid toxicity.
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Authors | Hongquan Wu, Que Zhu, Min Cai, Xin Tong, Dichuan Liu, Jing Huang, Gang Yang, Yonghong Jiang |
Journal | Cardiology
(Cardiology)
Vol. 127
Issue 4
Pg. 236-44
( 2014)
ISSN: 1421-9751 [Electronic] Switzerland |
PMID | 24480796
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- MicroRNAs
- Phosphates
- Carboxy-Lyases
- malonyl-CoA decarboxylase
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Topics |
- Animals
- Base Sequence
- Carboxy-Lyases
(antagonists & inhibitors)
- Gene Transfer Techniques
- Genetic Therapy
- HEK293 Cells
- Heart Failure
(etiology, pathology, therapy)
- Heart Function Tests
- Humans
- Male
- MicroRNAs
(pharmacology, therapeutic use)
- Microbubbles
- Molecular Sequence Data
- Myocardial Infarction
(complications, pathology)
- Myocardium
(metabolism, pathology)
- Phosphates
(metabolism)
- Plasmids
- RNA Interference
- Rats
- Rats, Wistar
- Ventricular Remodeling
(drug effects)
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