Circulating oncometabolite 2-hydroxyglutarate is a potential surrogate biomarker in patients with isocitrate dehydrogenase-mutant intrahepatic cholangiocarcinoma.

Abstract

PURPOSE:

Mutations in the IDH1 and IDH2 (IDH1/2) genes occur in approximately 20% of intrahepatic cholangiocarcinoma and lead to accumulation of 2-hydroxyglutarate (2HG) in the tumor tissue. However, it remains unknown whether IDH1/2 mutations can lead to high levels of 2HG circulating in the blood and whether serum 2HG can be used as a biomarker for IDH1/2 mutational status and tumor burden in intrahepatic cholangiocarcinoma.

EXPERIMENTAL DESIGN:

We initially measured serum 2HG concentration in blood samples collected from 31 patients with intrahepatic cholangiocarcinoma in a screening cohort. Findings were validated across 38 resected patients with intrahepatic cholangiocarcinoma from a second cohort with tumor volume measures. Circulating levels of 2HG were evaluated relative to IDH1/2 mutational status, tumor burden, and a number of clinical variables.

RESULTS:

Circulating levels of 2HG in the screening cohort were significantly elevated in patients with IDH1/2-mutant (median, 478 ng/mL) versus IDH1/2-wild-type (median, 118 ng/mL) tumors (P < 0.001). This significance was maintained in the validation cohort (343 ng/mL vs. 55 ng/mL, P < 0.0001) and levels of 2HG directly correlated with tumor burden in IDH1/2-mutant cases (P < 0.05). Serum 2HG levels ≥170 ng/mL could predict the presence of an IDH1/2 mutation with a sensitivity of 83% and a specificity of 90%. No differences were noted between the allelic variants IDH1 or IDH2 in regard to the levels of circulating 2HG.

CONCLUSIONS:

This study indicates that circulating 2HG may be a surrogate biomarker of IDH1 or IDH2 mutation status in intrahepatic cholangiocarcinoma and that circulating 2HG levels may correlate directly with tumor burden. Clin Cancer Res; 20(7); 1884-90. ©2014 AACR.

• Authors: Darrell R Borger, Lipika Goyal, Thomas Yau, Ronnie T Poon, Marek Ancukiewicz, Vikram Deshpande, David C Christiani, Hannah M Liebman, Hua Yang, Hyeryun Kim, Katharine Yen, Jason E Faris, A John Iafrate, Eunice L Kwak, Jeffrey W Clark, Jill N Allen, Lawrence S Blaszkowsky, Janet E Murphy, Supriya K Saha, Theodore S Hong, Jennifer Y Wo, Cristina R Ferrone, Kenneth K Tanabe, Nabeel Bardeesy, Kimberly S Straley, Sam Agresta, David P Schenkein, Leif W Ellisen, David P Ryan, Andrew X Zhu
• Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 20 Issue 7 Pg. 1884-90 (Apr 01 2014) ISSN: 1557-3265 [Electronic] United States
• PMID: 24478380 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Biomarkers, Tumor
• Glutarates
• alpha-hydroxyglutarate
• IDH2 protein, human
• Isocitrate Dehydrogenase
• IDH1 protein, human

Topics

• Adult
• Aged
• Bile Duct Neoplasms (blood, genetics, pathology)
• Bile Ducts, Intrahepatic (pathology)
• Biomarkers, Tumor (blood)
• Cholangiocarcinoma (blood, genetics, pathology)
• Female
• Glutarates (blood)
• Humans
• Isocitrate Dehydrogenase (blood, genetics)
• Male
• Middle Aged
• Mutation