Analgesic and anti-inflammatory activities of the 2,8-dihydroxy-1,6-dimethoxyxanthone from Haploclathra paniculata (Mart) Benth (Guttiferae).

Abstract

In the present study, the pharmacological effects of 2,8-dihydroxy-1,6-dimethoxyxanthone from the bark of Haploclathra paniculata were investigated in mice using in vivo inflammation and nociception models. Acetic acid-induced writhing, paw licking induced by formalin, hot plate, and carrageenan-induced paw edema tests were used to investigate the anti-inflammatory and antinociceptive activities of the xanthone compound. Xanthone, at both doses, inhibited abdominal writhing and the formalin test. At a dose of 20 mg/kg, the time of reaction to the hot plate increased, and significant effects were observed after 30, 60 and 90 min of treatment. At doses of 10 and 20 mg/kg p.o., the 2,8-dihydroxy-1,6-dimethoxyxanthone significantly reduced paw edema at 3 h after the stimulus. The tests also showed no acute toxicity of the xanthone compound in mice. 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability was also studied and confirmed the antioxidant activity of the xanthone. To propose the mechanism of action of anti-inflammatory activity of the xanthone, a molecular docking was performed using the isoenzymes cyclooxygenase 1 and 2 and the results indicate that the molecule is capable of inhibiting both the enzymes. Therefore, it can be concluded that 2,8-dihydroxy-1,6-dimethoxyxanthone from H. paniculata demonstrates analgesic, anti-inflammatory, and antioxidant activities.

• Authors: Maria Eliza Castro Moreira, Rosemary Gualberto Fonseca Alvarenga Pereira, Marcelo Jose Dias Silva, Danielle Ferreira Dias, Vanessa Silva Gontijo, Alexandre Giusti-Paiva, Marcia Paranho Veloso, Antônio Carlos Doriguetto, Tanus Jorge Nagem, Marcelo Henrique dos Santos
• Journal: Journal of medicinal food (J Med Food) Vol. 17 Issue 6 Pg. 686-93 (Jun 2014) ISSN: 1557-7600 [Electronic] United States
• PMID: 24476225 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 2,8-dihydroxy-1,6-dimethoxyxanthone
• Analgesics
• Anti-Inflammatory Agents
• Antioxidants
• Biphenyl Compounds
• Cyclooxygenase 2 Inhibitors
• Picrates
• Plant Extracts
• Xanthones
• Carrageenan
• 1,1-diphenyl-2-picrylhydrazyl
• Prostaglandin-Endoperoxide Synthases
• Acetic Acid

Topics

• Acetic Acid
• Analgesics (pharmacology, therapeutic use)
• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Antioxidants (pharmacology, therapeutic use)
• Biphenyl Compounds (metabolism)
• Carrageenan
• Clusiaceae (chemistry)
• Cyclooxygenase 2 Inhibitors (pharmacology, therapeutic use)
• Edema (chemically induced, drug therapy)
• Hot Temperature
• Inflammation (chemically induced, drug therapy)
• Male
• Pain (chemically induced, drug therapy)
• Pain Measurement
• Phytotherapy
• Picrates (metabolism)
• Plant Bark
• Plant Extracts (pharmacology, therapeutic use)
• Prostaglandin-Endoperoxide Synthases (metabolism)
• Rats, Wistar
• Xanthones (pharmacology, therapeutic use)