Hyperbaric oxygen preconditioning induces tolerance against oxidative injury and oxygen-glucose deprivation by up-regulating heat shock protein 32 in rat spinal neurons.

Abstract

OBJECTIVE:

Hyperbaric oxygen (HBO) preconditioning (HBO-PC) has been testified to have protective effects on spinal cord injury (SCI). However, the mechanisms remain enigmatic. The present study aimed to explore the effects of HBO-PC on primary rat spinal neurons against oxidative injury and oxygen-glucose deprivation (OGD) and the relationship with heat shock proteins (HSPs).

METHODS:

Primary rat spinal neurons after 7 days of culture were used in this study. HSPs were detected in rat spinal neurons following a single exposure to HBO at different time points by Western blot. Using lactate dehydrogenase release assay and cell counting kit-8 assay, the injuries induced by hydrogen peroxide (H2O2) insult or OGD were determined and compared among neurons treated with HBO-PC with or without HSP inhibitors.

RESULTS:

The results of Western blot showed that HSP27, HSP70 and HSP90 have a slight but not significant increase in primary neurons following HBO exposure. However, HSP32 expression significantly increased and reached highest at 12 h following HBO exposure. HBO-PC significantly increased the cell viability and decreased the medium lactate dehydrogenase content in cultures treated with H2O2 or OGD. Pretreatment with zinc protoporphyrin IX, a specific inhibitor of HSP32, significantly blocked the protective effects of HBO-PC.

CONCLUSIONS:

These results suggest that HBO-PC could protect rat spinal neurons in vitro against oxidative injury and OGD mostly by up-regulating of HSP32 expression.

• Authors: Guoyang Huang, Jiajun Xu, Li Xu, Shifeng Wang, Runping Li, Kan Liu, Juan Zheng, Zhiyu Cai, Kun Zhang, Yuandeng Luo, Weigang Xu
• Journal: PloS one (PLoS One) Vol. 9 Issue 1 Pg. e85967 ( 2014) ISSN: 1932-6203 [Electronic] United States
• PMID: 24465817 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Heme Oxygenase (Decyclizing)
• Hmox1 protein, rat
• Glucose
• Oxygen

Topics

• Animals
• Cells, Cultured
• Glucose (deficiency)
• Heme Oxygenase (Decyclizing) (metabolism)
• Hyperbaric Oxygenation
• Hypoxia (pathology)
• Neurons (drug effects, metabolism)
• Oxidative Stress (drug effects)
• Oxygen (pharmacology)
• Rats
• Spinal Cord Injuries (metabolism, pathology)
• Spine (drug effects, pathology)
• Time Factors
• Up-Regulation (drug effects)