Local and systemic immune mechanisms underlying the anti-colitis effects of the dairy bacterium Lactobacillus delbrueckii.

Abstract

Several probiotic bacteria have been proposed for treatment or prevention of inflammatory bowel diseases (IBD), showing a protective effect in animal models of experimental colitis and for some of them also in human clinical trials. While most of these probiotic bacteria are isolated from the digestive tract, we recently reported that a Lactobacillus strain isolated from cheese, L. delbrueckii subsp. lactis CNRZ327 (Lb CNRZ327), also possesses anti-inflammatory effects in vitro and in vivo, demonstrating that common dairy bacteria may be useful in the treatment or prevention of IBD. Here, we studied the mechanisms underlying the protective effects of Lb CNRZ327 in vivo, in a mouse dextran sodium sulfate (DSS) colitis model. During colitis, Lb CNRZ327 modulated the production of TGF-β, IL-6, and IL-12 in colonic tissue and of TGF-β and IL-6 in the spleen, and caused an expansion of CD4+Foxp3+ regulatory T cells in the cecal lymph nodes. Moreover, a strong tendency to CD4+Foxp3+ expansion was also observed in the spleen. The results of this study for the first time show that orally administered dairy lactobacilli can not only modulate mucosal but also systemic immune responses and constitute an effective treatment of IBD.

• Authors: Clarissa Santos Rocha, Ana Cristina Gomes-Santos, Thais Garcias Moreira, Marcela de Azevedo, Tessalia Diniz Luerce, Mahendra Mariadassou, Ana Paula Longaray Delamare, Philippe Langella, Emmanuelle Maguin, Vasco Azevedo, Ana Maria Caetano de Faria, Anderson Miyoshi, Maarten van de Guchte
• Journal: PloS one (PLoS One) Vol. 9 Issue 1 Pg. e85923 ( 2014) ISSN: 1932-6203 [Electronic] United States
• PMID: 24465791 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Immunoglobulin A, Secretory
• Dextran Sulfate

Topics

• Animals
• Anti-Inflammatory Agents (immunology)
• Body Weight
• CD4-Positive T-Lymphocytes (immunology)
• Cecum (immunology, microbiology)
• Cell Differentiation
• Colitis (chemically induced, immunology, microbiology, pathology)
• Colon (immunology, microbiology, pathology)
• Cytokines (biosynthesis)
• Dairying
• Dextran Sulfate
• Female
• Humans
• Immunoglobulin A, Secretory (metabolism)
• Lactobacillus delbrueckii (immunology)
• Lymph Nodes (immunology)
• Mice
• Mice, Inbred C57BL
• Spleen (metabolism)