Abstract | OBJECTIVE: METHODS: Twenty-seven rats were randomized into 3 equal groups and subjected to sham operation (group A), spinal cord ischemia- reperfusion injury (group B), or ischemia-reperfusion injury with adenosine treatment. Spinal cord ischemia- reperfusion injury was induced by cross-clamping of the abdominal aorta inferior to the left renal artery. The spinal cord function was assessed using the Modified Tarlov Scale at 6, 12, and 24 h after reperfusion. At 24 h after reperfusion, histological analysis was carried out with HE staining; cell apoptosis and viability were determined with TUNEL staining, and the expressions of GRP-78 and caspase-12 proteins were determined with Western blotting. RESULTS: HE staining of the spinal cord showed extensive spinal cord injury such as cell edema in group B as compared with group C. Compared with group A, group B showed a significantly increased number of apoptotic cells; the number of apoptotic cells in group B was greater than that in group C. Compared with group B, group C showed significantly increased GRP-78 expression (P<0.01) and decreased caspase-12 expression (P<0.01). CONCLUSION:
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Authors | Qi Fu, Zhengfang Zhou, Xiaohui Li, Huiming Guo, Xiaoping Fan, Jimei Chen, Jian Zhuang, Shaoyi Zheng, Ping Zhu |
Journal | Nan fang yi ke da xue xue bao = Journal of Southern Medical University
(Nan Fang Yi Ke Da Xue Xue Bao)
Vol. 34
Issue 1
Pg. 92-5
(Jan 2014)
ISSN: 1673-4254 [Print] China |
PMID | 24463125
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- GRP78 protein, rat
- Heat-Shock Proteins
- Casp12 protein, rat
- Caspase 12
- Adenosine
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Topics |
- Adenosine
(pharmacology)
- Animals
- Apoptosis
(drug effects)
- Caspase 12
(metabolism)
- Heat-Shock Proteins
(metabolism)
- Ischemic Preconditioning
(methods)
- Male
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(metabolism)
- Spinal Cord Ischemia
(metabolism)
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