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[Protective effect of adenosine preconditioning against spinal cord ischemia-reperfusion injury in rats].

AbstractOBJECTIVE:
To study the effect of adenosine preconditioning on cell apoptosis and expressions of glucose-regulated protein (GRP-78) and cysteinyl aspartate-specific protease 12 (caspase-12) in rats with spinal cord ischemia-reperfusion injury.
METHODS:
Twenty-seven rats were randomized into 3 equal groups and subjected to sham operation (group A), spinal cord ischemia-reperfusion injury (group B), or ischemia-reperfusion injury with adenosine treatment. Spinal cord ischemia-reperfusion injury was induced by cross-clamping of the abdominal aorta inferior to the left renal artery. The spinal cord function was assessed using the Modified Tarlov Scale at 6, 12, and 24 h after reperfusion. At 24 h after reperfusion, histological analysis was carried out with HE staining; cell apoptosis and viability were determined with TUNEL staining, and the expressions of GRP-78 and caspase-12 proteins were determined with Western blotting.
RESULTS:
HE staining of the spinal cord showed extensive spinal cord injury such as cell edema in group B as compared with group C. Compared with group A, group B showed a significantly increased number of apoptotic cells; the number of apoptotic cells in group B was greater than that in group C. Compared with group B, group C showed significantly increased GRP-78 expression (P<0.01) and decreased caspase-12 expression (P<0.01).
CONCLUSION:
Adenosine can up-regulate GRP-78 expression and down-regulate caspase-12 expression, and protects the spinal cord against ischemia-reperfusion injury by inhibiting cell apoptosis.
AuthorsQi Fu, Zhengfang Zhou, Xiaohui Li, Huiming Guo, Xiaoping Fan, Jimei Chen, Jian Zhuang, Shaoyi Zheng, Ping Zhu
JournalNan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 34 Issue 1 Pg. 92-5 (Jan 2014) ISSN: 1673-4254 [Print] China
PMID24463125 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • GRP78 protein, rat
  • Heat-Shock Proteins
  • Casp12 protein, rat
  • Caspase 12
  • Adenosine
Topics
  • Adenosine (pharmacology)
  • Animals
  • Apoptosis (drug effects)
  • Caspase 12 (metabolism)
  • Heat-Shock Proteins (metabolism)
  • Ischemic Preconditioning (methods)
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury (metabolism)
  • Spinal Cord Ischemia (metabolism)

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