Abstract |
Mesenchymal stem cells (MSC) are present in most, if not all, tissues and are believed to contribute to tissue regeneration and the tissue immune microenvironment. Murine MSCs exert immunosuppressive effects through production of inducible nitric oxide synthase (iNOS), whereas human MSCs use indoleamine 2,3-dioxygenase (IDO). Thus, studies of MSC-mediated immunomodulation in mice may not be informative in the setting of human disease, although this critical difference has been mainly ignored. To address this issue, we established a novel humanized system to model human MSCs, using murine iNOS(-/-) MSCs that constitutively or inducibly express an ectopic human IDO gene. In this system, inducible IDO expression is driven by a mouse iNOS promoter that can be activated by inflammatory cytokine stimulation in a similar fashion as the human IDO promoter. These IDO-expressing humanized MSCs (MSC-IDO) were capable of suppressing T-lymphocyte proliferation in vitro. In melanoma and lymphoma tumor models, MSC-IDO promoted tumor growth in vivo, an effect that was reversed by the IDO inhibitor 1-methyl-tryptophan. We found that MSC-IDO dramatically reduced both tumor-infiltrating CD8(+) T cells and B cells. Our findings offer an important new line of evidence that interventional targeting of IDO activity could be used to restore tumor immunity in humans, by relieving IDO-mediated immune suppression of MSCs in the tumor microenvironment as well as in tumor cells themselves.
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Authors | Weifang Ling, Jimin Zhang, Zengrong Yuan, Guangwen Ren, Liying Zhang, Xiaodong Chen, Arnold B Rabson, Arthur I Roberts, Ying Wang, Yufang Shi |
Journal | Cancer research
(Cancer Res)
Vol. 74
Issue 5
Pg. 1576-87
(Mar 01 2014)
ISSN: 1538-7445 [Electronic] United States |
PMID | 24452999
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | ©2014 AACR |
Chemical References |
- Indoleamine-Pyrrole 2,3,-Dioxygenase
- Nitric Oxide Synthase Type II
- Nos2 protein, mouse
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Topics |
- Animals
- B-Lymphocytes
(immunology, metabolism)
- CD8-Positive T-Lymphocytes
(immunology, metabolism)
- Cell Proliferation
- Cells, Cultured
- Humans
- Indoleamine-Pyrrole 2,3,-Dioxygenase
(genetics, immunology, metabolism)
- Mesenchymal Stem Cells
(immunology, metabolism)
- Mice
- Mice, Inbred C57BL
- Nitric Oxide Synthase Type II
(genetics, immunology, metabolism)
- Promoter Regions, Genetic
(genetics, immunology)
- Tumor Microenvironment
(genetics, immunology)
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