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The effect of bevacizumab and 5-Fluorouracil combination on epidural fibrosis in a rat laminectomy model.

AbstractOBJECTIVE:
An animal model of laminectomy in rats was used to study scar tissue formation around the spinal cord. Bevacizumab (BV) [a recombinant, humanized, monoclonal antibody targeting vascular endothelial growth factor], 5-Fluorouracil (5-FU) and BV+5-FU was tested in this system for its ability to decrease fibrous tissue formation.
MATERIALS AND METHODS:
Twenty-eight Sprague Dawley rats were used in this sudy. Rats were divided into four groups; a control group, a BV group, a 5-FU group and a BV+5-FU group. L1-2 laminectomies were performed on the rats. The medicated groups were treated with topical drug administration. After 6 weeks, the rats were sacrified and histologic sections prepared from the spines were examined and graded by a pathologist. Epidural fibrosis and fibroblast density were evaluated under light microscope.
RESULTS:
BV (Avastin: Genentech, San Francisco, CA, USA) significantly reduced the density of the scar tissue undermining the laminas (p < 0.005). Monotherapy with 5-FU did not change the scar formation in the back (p = 0.317). Combination of 5-FU and BV was more effective on reducing the epidural fibrosis after laminectomy on rats (p < 0.001).
CONCLUSIONS:
Bevacizumab reduced the spinal epidural fibrosis significantly that developed in rats after laminectomy and 5-Fluorouracil combination had a synergic effect. Further investigations under the light of these findings may help to reduce epidural fibrosis formation after laminectomy.
AuthorsU Ozkan, A Osun, A Samancioglu, S Ercan, U Firat, S Kemaloglu
JournalEuropean review for medical and pharmacological sciences (Eur Rev Med Pharmacol Sci) Vol. 18 Issue 1 Pg. 95-100 ( 2014) ISSN: 2284-0729 [Electronic] Italy
PMID24452949 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Monoclonal, Humanized
  • Vascular Endothelial Growth Factor A
  • Bevacizumab
  • Fluorouracil
Topics
  • Animals
  • Antibodies, Monoclonal, Humanized (therapeutic use)
  • Bevacizumab
  • Drug Therapy, Combination
  • Epidural Space (drug effects, pathology)
  • Fibrosis
  • Fluorouracil (therapeutic use)
  • Laminectomy (adverse effects)
  • Lumbar Vertebrae (surgery)
  • Male
  • Postoperative Complications (drug therapy)
  • Rats, Sprague-Dawley
  • Vascular Endothelial Growth Factor A (antagonists & inhibitors)

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