Abstract |
A two-step diagnostic algorithm is recommended to detect Clostridium difficile infections; however, samples are regularly found that are glutamate dehydrogenase (GDH) positive but stool toxin negative. In the present single-centre prospective study we focused on these 'difficult-to-interpret' samples and characterized them by anaerobic culture, toxigenic culture, slpA sequence typing and multiplex PCR (GenoType CDiff). The majority of stool toxin A and B-negative samples have been caused by toxigenic strains including ribotype 027. The multiplex PCR was faster and more sensitive compared with culture and allowed preliminary identification of hypervirulent strains in stool samples on the same day.
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Authors | J Stahlmann, M Schönberg, M Herrmann, L von Müller |
Journal | Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
(Clin Microbiol Infect)
Vol. 20
Issue 9
Pg. O590-2
(Sep 2014)
ISSN: 1469-0691 [Electronic] England |
PMID | 24450741
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 The Authors Clinical Microbiology and Infection © 2014 European Society of Clinical Microbiology and Infectious Diseases. |
Chemical References |
- Bacterial Proteins
- Bacterial Toxins
- Enterotoxins
- tcdA protein, Clostridium difficile
- toxB protein, Clostridium difficile
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Topics |
- Bacterial Proteins
(analysis, genetics)
- Bacterial Toxins
(analysis, genetics)
- Clostridioides difficile
(classification, genetics, isolation & purification)
- Clostridium Infections
(diagnosis, microbiology)
- Enterotoxins
(analysis, genetics)
- Feces
(microbiology)
- Humans
- Multiplex Polymerase Chain Reaction
(methods)
- Prospective Studies
- Ribotyping
- Time Factors
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