Abstract |
The objective of this study was to develop chitosan-based delivery of cobalt oxide nanoparticles to human leukemic cells and investigate their specific induction of apoptosis. The physicochemical properties of the chitosan-coated cobalt oxide nanoparticles were characterized using transmission electron microscopy, dynamic light scattering, X-ray diffraction, and Fourier transform infrared spectroscopy. The solubility of chitosan-coated cobalt oxide nanoparticles was higher at acidic pH, which helps to release more cobalt ions into the medium. Chitosan-coated cobalt oxide nanoparticles showed good compatibility with normal cells. However, our results showed that exposure of leukemic cells (Jurkat cells) to chitosan-coated cobalt oxide nanoparticles caused an increase in reactive oxygen species generation that was abolished by pretreatment of cells with the reactive oxygen species scavenger N-acetyl-L-cysteine. The apoptosis of Jurkat cells was confirmed by flow-cytometric analysis. Induction of TNF-α secretion was observed from stimulation of Jurkat cells with chitosan-coated cobalt oxide nanoparticles. We also tested the role of TNF-α in the induction of Jurkat cell death in the presence of TNF-α and caspase inhibitors. Treatment of leukemic cells with a blocker had a greater effect on cancer cell viability. From our findings, oxidative stress and caspase activation are involved in cancer cell death induced by chitosan-coated cobalt oxide nanoparticles.
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Authors | Sourav Chattopadhyay, Sandeep Kumar Dash, Santanu Kar Mahapatra, Satyajit Tripathy, Totan Ghosh, Balaram Das, Debasis Das, Panchanan Pramanik, Somenath Roy |
Journal | Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
(J Biol Inorg Chem)
Vol. 19
Issue 3
Pg. 399-414
(Mar 2014)
ISSN: 1432-1327 [Electronic] Germany |
PMID | 24445996
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Oxides
- Tumor Necrosis Factor-alpha
- Cobalt
- Chitosan
- cobalt oxide
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Topics |
- Apoptosis
(drug effects, physiology)
- Chitosan
(chemistry, pharmacology, therapeutic use)
- Cobalt
(chemistry, pharmacology, therapeutic use)
- Dose-Response Relationship, Drug
- Humans
- Jurkat Cells
- Leukemia, T-Cell
(drug therapy, metabolism)
- Lymphocytes
(drug effects, metabolism)
- Metal Nanoparticles
(chemistry)
- Oxides
(chemistry, pharmacology, therapeutic use)
- Tumor Necrosis Factor-alpha
(pharmacology, physiology)
- X-Ray Diffraction
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