HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Proteome and allergenome of Asian wasp, Vespa affinis, venom and IgE reactivity of the venom components.

Abstract
Vespa affinis (Asian wasp, Thai banded tiger wasp, or local name: Tor Hua Seua) causes the most frequent incidence of medically important Hymenoptera sting in South and Southeast Asia. However, data on the venom components attributable to the sting derived-clinical manifestations (local reactions, IgE mediated-anaphylaxis, or systemic envenomation) are lacking. This study provides the first set information on V. affinis venom proteome, allergenome, and IgE reactivity of individual venom components. From 2DE-gel based-proteomics, the venom revealed 93 protein spots, of which proteins in 51 spots could be identified and classified into three groups: typical venom components and structural and housekeeping proteins. Venom proteins in 32 spots reacted with serum IgE of wasp allergic patients. Major allergenic proteins that reacted to IgE of >50% of the wasp allergic patients included PLA1 (100%), arginine kinase (73%), heat shock 70 kDa protein (73.3%), venom allergen-5 (66.7%), enolase (66.7%), PLA1 magnifin (60%), glyceraldehyde-3-phosphate dehydrogenase (60%), hyaluronidase (53.3%), and fructose-bisphosphate aldolase (53.3%). The venom minor allergens were GB17876 transcript (40%), GB17291 transcript (20%), malic enzyme (13.3%), aconitate hydratase (6.7%), and phosphoglucomutase (6.7%). The information has diagnostic and clinical implications for future improvement of case diagnostic sensitivity and specificity, component-resolve diagnosis, and design of specific Hymenoptera venom immunotherapy.
AuthorsNitat Sookrung, Siriporn Wong-din-Dam, Anchalee Tungtrongchitr, Onrapak Reamtong, Nitaya Indrawattana, Yuwaporn Sakolvaree, Nualanong Visitsunthorn, Wiparat Manuyakorn, Wanpen Chaicumpa
JournalJournal of proteome research (J Proteome Res) Vol. 13 Issue 3 Pg. 1336-44 (Mar 07 2014) ISSN: 1535-3907 [Electronic] United States
PMID24437991 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Allergens
  • Insect Proteins
  • Proteome
  • Wasp Venoms
  • Immunoglobulin E
Topics
  • Adolescent
  • Adult
  • Allergens (immunology, isolation & purification)
  • Anaphylaxis (blood, immunology, physiopathology)
  • Animals
  • Child
  • Female
  • Humans
  • Immunoglobulin E (blood)
  • Insect Bites and Stings (blood, immunology, physiopathology)
  • Insect Proteins (immunology, isolation & purification)
  • Male
  • Molecular Sequence Annotation
  • Protein Binding
  • Proteome (immunology, isolation & purification)
  • Wasp Venoms (chemistry)
  • Wasps (physiology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: