Abstract |
Vespa affinis (Asian wasp, Thai banded tiger wasp, or local name: Tor Hua Seua) causes the most frequent incidence of medically important Hymenoptera sting in South and Southeast Asia. However, data on the venom components attributable to the sting derived-clinical manifestations (local reactions, IgE mediated- anaphylaxis, or systemic envenomation) are lacking. This study provides the first set information on V. affinis venom proteome, allergenome, and IgE reactivity of individual venom components. From 2DE-gel based-proteomics, the venom revealed 93 protein spots, of which proteins in 51 spots could be identified and classified into three groups: typical venom components and structural and housekeeping proteins. Venom proteins in 32 spots reacted with serum IgE of wasp allergic patients. Major allergenic proteins that reacted to IgE of >50% of the wasp allergic patients included PLA1 (100%), arginine kinase (73%), heat shock 70 kDa protein (73.3%), venom allergen-5 (66.7%), enolase (66.7%), PLA1 magnifin (60%), glyceraldehyde-3-phosphate dehydrogenase (60%), hyaluronidase (53.3%), and fructose-bisphosphate aldolase (53.3%). The venom minor allergens were GB17876 transcript (40%), GB17291 transcript (20%), malic enzyme (13.3%), aconitate hydratase (6.7%), and phosphoglucomutase (6.7%). The information has diagnostic and clinical implications for future improvement of case diagnostic sensitivity and specificity, component-resolve diagnosis, and design of specific Hymenoptera venom immunotherapy.
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Authors | Nitat Sookrung, Siriporn Wong-din-Dam, Anchalee Tungtrongchitr, Onrapak Reamtong, Nitaya Indrawattana, Yuwaporn Sakolvaree, Nualanong Visitsunthorn, Wiparat Manuyakorn, Wanpen Chaicumpa |
Journal | Journal of proteome research
(J Proteome Res)
Vol. 13
Issue 3
Pg. 1336-44
(Mar 07 2014)
ISSN: 1535-3907 [Electronic] United States |
PMID | 24437991
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Allergens
- Insect Proteins
- Proteome
- Wasp Venoms
- Immunoglobulin E
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Topics |
- Adolescent
- Adult
- Allergens
(immunology, isolation & purification)
- Anaphylaxis
(blood, immunology, physiopathology)
- Animals
- Child
- Female
- Humans
- Immunoglobulin E
(blood)
- Insect Bites and Stings
(blood, immunology, physiopathology)
- Insect Proteins
(immunology, isolation & purification)
- Male
- Molecular Sequence Annotation
- Protein Binding
- Proteome
(immunology, isolation & purification)
- Wasp Venoms
(chemistry)
- Wasps
(physiology)
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