Therapeutic effect of anti-αv integrin mAb on Theiler's murine encephalomyelitis virus-induced demyelinating disease.

Abstract

We examined the regulatory role of αv integrins in the development of Theiler's murine encephalomyelitis virus-induced demyelinating disease (TMEV-IDD), a model of multiple sclerosis (MS). Blockade of αv integrins by anti-αv integrin monoclonal antibody (mAb) in the effector phase significantly suppressed the development of TMEV-IDD both clinically and histologically. The number of infiltrating mononuclear cells (MNCs) in the CNS was significantly decreased in mice treated with anti-αv integrin mAb. Flow cytometric analysis of cytokine staining revealed that absolute numbers of IFN-γ- and IL-17-producing CD4+ and IFN-γ-producing CD8+ T cells were significantly decreased in the CNS of mice treated with anti-αv integrin mAb. These data suggest that αv integrins may play important roles in the development of TMEV-IDD.

• Authors: Hiroki Tomiki, Tomoki Kaneyama, Kunitoshi Kobayashi, Yuji Inaba, Motoki Ichikawa, Hideo Yagita, Byung S Kim, Chang-Sung Koh
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 268 Issue 1-2 Pg. 25-34 (Mar 15 2014) ISSN: 1872-8421 [Electronic] Netherlands
• PMID: 24434077 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies, Monoclonal
• Integrin alphaV

Topics

• Animals
• Antibodies, Monoclonal (pharmacology)
• Cardiovirus Infections (immunology, pathology)
• Demyelinating Autoimmune Diseases, CNS (immunology, pathology)
• Female
• Flow Cytometry
• Fluorescent Antibody Technique
• Integrin alphaV (immunology)
• Mice
• Real-Time Polymerase Chain Reaction
• Theilovirus