Aberrant expression of monocarboxylate transporter 4 in tumour cells predicts an unfavourable outcome in patients with hepatocellular carcinoma.

Abstract

BACKGROUND & AIMS:

The tumour cell microenvironment, which includes local oxygen saturation, pericellular pH and stromal cells, can modulate tumour progression. This study determined the prognostic impact of infiltrating tumour-associated macrophages and the expression of monocarboxylate transporter 4 (MCT4) and glypican 3 (GPC3) in hepatocellular carcinoma (HCC) clinical specimens.

METHODS:

A total of 225 cases of resected HCC were subjected to immunohistochemical analyses of CD68, CD204, MCT4 and GPC3. Immunoreactivities and other common clinicopathological parameters were subjected to univariate prognostic analyses for overall survival (OS, n = 225) and disease-free survival (DFS, n = 222). All variables with prognostic impact were further analysed in multivariate analysis.

RESULTS:

Increased intratumoural infiltration of CD204-positive or MCT4-positive macrophages suggested shorter OS (P = 0.015 or P = 0.001 respectively), but DFS was not altered. The GPC3 score (with an emphasis on circumferential immunoreactivity) was correlated with shorter OS and DFS. Aberrant expression of MCT4 in HCC cells was observed in a subset of HCC cases (21%, 47/225). In those cases, significantly poorer OS (P < 0.0001) and DFS (P = 0.0003) were observed, and there was a positive correlation with the intratumoural infiltration of CD204- or MCT4-positive macrophages and the GPC3 score. Multivariate analysis showed that aberrant MCT4 expression in HCC cells was an independent prognostic factor for shorter OS (P = 0.018) and DFS (P = 0.006) after resection of HCC.

CONCLUSION:

Aberrant expression of MCT4 in carcinoma cells serves as a novel, independent prognostic factor for HCC, indicating a poorer patient outcome.

• Authors: Akinobu Ohno, Kenji Yorita, Yukihiro Haruyama, Kazuhiro Kondo, Atsuhiko Kato, Toshihiko Ohtomo, Makiko Kawaguchi, Kousuke Marutuska, Kazuo Chijiiwa, Hiroaki Kataoka
• Journal: Liver international : official journal of the International Association for the Study of the Liver (Liver Int) Vol. 34 Issue 6 Pg. 942-52 (Jul 2014) ISSN: 1478-3231 [Electronic] United States
• PMID: 24433439 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Biomarkers, Tumor
• Monocarboxylic Acid Transporters
• Muscle Proteins
• SLC16A4 protein, human

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (analysis)
• Carcinoma, Hepatocellular (chemistry, mortality, pathology, therapy)
• Chi-Square Distribution
• Disease-Free Survival
• Female
• Humans
• Immunohistochemistry
• Kaplan-Meier Estimate
• Liver Neoplasms (chemistry, mortality, pathology, therapy)
• Male
• Middle Aged
• Monocarboxylic Acid Transporters (analysis)
• Multivariate Analysis
• Muscle Proteins (analysis)
• Predictive Value of Tests
• Proportional Hazards Models
• Risk Factors
• Time Factors
• Treatment Outcome
• Young Adult