Eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis.

Abstract

Lemon (Citrus limon) contains various bioactive flavonoids, and prevents obesity and obesity-associated metabolic diseases. We focused on eriocitrin (eriodictyol 7-rutinoside), a powerful antioxidative flavonoid in lemon with lipid-lowering effects in a rat model of high-fat diet. To investigate the mechanism of action of eriocitrin, we conducted feeding experiments on zebrafish with diet-induced obesity. Oral administration of eriocitrin (32 mg/kg/day for 28 days) improved dyslipidaemia and decreased lipid droplets in the liver. DNA microarray analysis revealed that eriocitrin increased mRNA of mitochondrial biogenesis genes, such as mitochondria transcription factor, nuclear respiratory factor 1, cytochrome c oxidase subunit 4, and ATP synthase. In HepG2 cells, eriocitrin also induced the corresponding orthologues, and reduced lipid accumulation under conditions of lipid loading. Eriocitrin increased mitochondrial size and mtDNA content, which resulted in ATP production in HepG2 cells and zebrafish. In summary, dietary eriocitrin ameliorates diet-induced hepatic steatosis with activation of mitochondrial biogenesis.

• Authors: Masanori Hiramitsu, Yasuhito Shimada, Junya Kuroyanagi, Takashi Inoue, Takao Katagiri, Liqing Zang, Yuhei Nishimura, Norihiro Nishimura, Toshio Tanaka
• Journal: Scientific reports (Sci Rep) Vol. 4 Pg. 3708 (Jan 15 2014) ISSN: 2045-2322 [Electronic] England
• PMID: 24424211 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Mitochondrial
• Flavanones
• Flavonoids
• Lipids
• RNA, Messenger
• Adenosine Triphosphate
• eriocitrin

Topics

• Adenosine Triphosphate (metabolism)
• Animals
• Cell Line, Tumor
• Citrus (chemistry)
• DNA, Mitochondrial (genetics, metabolism)
• Diet, High-Fat (adverse effects)
• Fatty Liver (drug therapy, genetics, metabolism)
• Flavanones (pharmacology)
• Flavonoids (pharmacology)
• Hep G2 Cells
• Humans
• Lipids (physiology)
• Liver (drug effects)
• Mitochondria (drug effects, genetics, metabolism)
• Obesity (genetics, metabolism)
• Organelle Biogenesis
• RNA, Messenger (genetics)
• Rats
• Zebrafish