Abstract | BACKGROUND: METHODS: We evaluated 159 malignant effusions (84 adenocarcinomas and 75 malignant mesotheliomas). Claudin-4 immunohistochemistry was performed on cell-block paraffin sections and scored for staining intensity, staining pattern (cytoplasmic versus membranous), and percentage of positive tumor cells. Appropriate positive and negative controls were used throughout. RESULTS: All cases of mesothelioma were negative for claudin-4 (0 of 64). Eighty-three of 84 cases of adenocarcinoma were positive (99%); 1 case of serous carcinoma was negative. Most adenocarcinomas showed strong and diffuse membranous staining (71 of 84; 84%); 12 cases (14%) showed membranous staining of moderate intensity. The overall sensitivity for adenocarcinoma was 99% (83 of 84). CONCLUSIONS:
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Authors | Vickie Y Jo, Edmund S Cibas, Geraldine S Pinkus |
Journal | Cancer cytopathology
(Cancer Cytopathol)
Vol. 122
Issue 4
Pg. 299-306
(Apr 2014)
ISSN: 1934-6638 [Electronic] United States |
PMID | 24421209
(Publication Type: Comparative Study, Journal Article)
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Copyright | © 2014 American Cancer Society. |
Chemical References |
- Biomarkers, Tumor
- Claudin-4
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Topics |
- Adenocarcinoma
(chemistry, pathology)
- Aged
- Biomarkers, Tumor
(analysis)
- Biopsy, Needle
- Claudin-4
(analysis)
- Cytodiagnosis
(methods)
- Databases, Factual
- Diagnosis, Differential
- Female
- Humans
- Immunohistochemistry
- Male
- Mesothelioma
(chemistry, pathology)
- Middle Aged
- Pleural Effusion, Malignant
(chemistry, pathology)
- Reproducibility of Results
- Retrospective Studies
- Sensitivity and Specificity
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