Identification of the Ro and La antigens in the endoribonuclease VII--ribonucleoprotein complex.

Abstract

45 S RNP (ribonucleoprotein) particles from calf thymus or L5178y mouse lymphoma cells contain the poly(A)-modulated and oligo(U)-binding endoribonuclease VII [Bachmann, Zahn & Müller (1983) J. Biol. Chem. 258, 7033-7040]. From these particles a 4.5 S RNA was isolated that possesses an oligo(U) sequence. By using monospecific and non-cross-reacting antibodies directed against the La or Ro antigen, both proteins were identified in the endoribonuclease VII-RNP complex after phosphorylation in vitro. In a second approach, endoribonuclease VII activity was identified in immunoaffinity-purified Ro RNPs after preparative isoelectric focusing. Therefore we conclude that the 4.5 S RNA belongs to the Ro RNAs. The results indicate a possible function of endoribonuclease VII in activating stored mRNAs.

• Authors: M Bachmann, W J Mayet, H C Schröder, K Pfeifer, K H Meyer zum Büschenfelde, W E Müller
• Journal: The Biochemical journal (Biochem J) Vol. 243 Issue 1 Pg. 189-94 (Apr 01 1987) ISSN: 0264-6021 [Print] England
• PMID: 2440423 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Autoantigens
• Macromolecular Substances
• RNA, Small Cytoplasmic
• RO60 protein, human
• Ribonucleoproteins
• SS-A antigen
• SS-B antigen
• RNA
• Endoribonucleases
• endoribonuclease VII

Topics

• Animals
• Antibodies (immunology)
• Autoantigens (analysis)
• Chemical Precipitation
• Endoribonucleases (immunology)
• Macromolecular Substances
• Mice
• RNA (analysis)
• RNA, Small Cytoplasmic
• Ribonucleoproteins (analysis, immunology)