HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Analysis of HIV-1 protease gene reveals frequent multiple infections followed by recombination among drug treated individuals living in São Paulo and Santos, Brazil.

Abstract
The present study investigated the prevalence of HIV-1 multiple infections in a population composed by 47 patients under HAART failure and enrolled at the National DST/AIDS, Program, Ministry of Health, Brazil.Detection of multiple infections was done using a previously published RFLP assay for the HIV-1 protease gene, which is able of distinguishing between infections caused by a single or multiple HIV-1 subtypes. Samples with multiple infections were cloned, and sequence data submitted to phylogenetic analysis. We were able to identify 17 HIV-1 multiple infections out of 47 samples. Multiple infections were mostly composed by a mixture of recombinant viruses (94%), with only one case in which protease gene pure subtypes B and F were recovered. This is the first study that reports the prevalence of multiple infections and intersubtype recombinants in a population undergoing HAART in Brazil. Based on the data there was a steep increase of multiple infections after the introduction of the combined antiretroviral therapy in Brazil. Cases of multiple infections may be associated with HIV-1 genetic diversity through recombination allowing for the generation of viruses showing a combination of resistance mutations.
AuthorsEdsel Renata De Morais Nunes, Jean Paulo Zukurov, Juliana Terzi Maricato, Maria Cecília Araripe Sucupira, Ricardo Sobhie Diaz, Luíz Mário Ramos Janini
JournalPloS one (PLoS One) Vol. 9 Issue 1 Pg. e84066 ( 2014) ISSN: 1932-6203 [Electronic] United States
PMID24404149 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-HIV Agents
  • HIV Protease
Topics
  • Anti-HIV Agents (pharmacology)
  • Brazil
  • Drug Resistance, Viral
  • Genotype
  • HIV Infections (epidemiology, virology)
  • HIV Protease (genetics)
  • HIV-1 (classification, genetics)
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • Reassortant Viruses (classification, genetics)
  • Recombination, Genetic

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: