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Wilms' tumor 1 is involved in tumorigenicity of glioblastoma by regulating cell proliferation and apoptosis.

Abstract
The prognosis for patients with glioblastoma is very poor, despite intensive treatment, including surgery and chemoradiotherapy. Wilms' tumor 1 (WT1) is expressed in most glioblastoma samples, and immunotherapy targeting WT1 has proven to be effective in recurrent glioblastoma. However, the functional roles of WT1 in glioblastoma are not clear. To examine the functional roles of WT1 in glioblastoma, glioblastoma cell lines with reduced WT1 expression were generated using short hairpin RNA(shRNA)-expressing lentivirus. Proliferation of WT1-knockdown glioblastoma cells was significantly slower than control cells with high WT1 expression. In addition, apoptosis was increased in WT1-knockdown glioblastoma cells. Furthermore, WT1-knockdown glioblastoma cells, and control glioblastoma cells were intra-cranially injected into immunodeficient mice. In vivo tumor growth of WT1-knockdown glioblastoma cells was significantly reduced compared to control glioblastoma cells. These results show that WT1 is involved in glioblastoma cell proliferation and apoptosis and that this protein has oncogenic roles in glioblastoma.
AuthorsNoriyuki Kijima, Naoki Hosen, Naoki Kagawa, Naoya Hashimoto, Manabu Kinoshita, Yusuke Oji, Haruo Sugiyama, Toshiki Yoshimine
JournalAnticancer research (Anticancer Res) Vol. 34 Issue 1 Pg. 61-7 (Jan 2014) ISSN: 1791-7530 [Electronic] Greece
PMID24403445 (Publication Type: Journal Article)
Chemical References
  • Biomarkers, Tumor
  • DNA-Binding Proteins
  • RNA, Small Interfering
  • Rag2 protein, mouse
  • WT1 Proteins
Topics
  • Animals
  • Animals, Newborn
  • Apoptosis
  • Biomarkers, Tumor (genetics, metabolism)
  • Cell Proliferation
  • DNA-Binding Proteins (physiology)
  • Gene Expression Profiling
  • Glioblastoma (metabolism, mortality, pathology)
  • Humans
  • Immunoenzyme Techniques
  • Mice
  • Oligonucleotide Array Sequence Analysis
  • RNA, Small Interfering (genetics)
  • Survival Rate
  • Tumor Cells, Cultured
  • WT1 Proteins (antagonists & inhibitors, genetics, metabolism)
  • Xenograft Model Antitumor Assays

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