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Determining cell-of-origin subtypes of diffuse large B-cell lymphoma using gene expression in formalin-fixed paraffin-embedded tissue.

Abstract
The assignment of diffuse large B-cell lymphoma into cell-of-origin (COO) groups is becoming increasingly important with the emergence of novel therapies that have selective biological activity in germinal center B cell-like or activated B cell-like groups. The Lymphoma/Leukemia Molecular Profiling Project's Lymph2Cx assay is a parsimonious digital gene expression (NanoString)-based test for COO assignment in formalin-fixed paraffin-embedded tissue (FFPET). The 20-gene assay was trained using 51 FFPET biopsies; the locked assay was then validated using an independent cohort of 68 FFPET biopsies. Comparisons were made with COO assignment using the original COO model on matched frozen tissue. In the validation cohort, the assay was accurate, with only 1 case with definitive COO being incorrectly assigned, and robust, with >95% concordance of COO assignment between 2 independent laboratories. These qualities, along with the rapid turnaround time, make Lymph2Cx attractive for implementation in clinical trials and, ultimately, patient management.
AuthorsDavid W Scott, George W Wright, P Mickey Williams, Chih-Jian Lih, William Walsh, Elaine S Jaffe, Andreas Rosenwald, Elias Campo, Wing C Chan, Joseph M Connors, Erlend B Smeland, Anja Mottok, Rita M Braziel, German Ott, Jan Delabie, Raymond R Tubbs, James R Cook, Dennis D Weisenburger, Timothy C Greiner, Betty J Glinsmann-Gibson, Kai Fu, Louis M Staudt, Randy D Gascoyne, Lisa M Rimsza
JournalBlood (Blood) Vol. 123 Issue 8 Pg. 1214-7 (Feb 20 2014) ISSN: 1528-0020 [Electronic] United States
PMID24398326 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References
  • Fixatives
  • Formaldehyde
Topics
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biopsy
  • Cell Lineage (genetics)
  • Female
  • Fixatives
  • Formaldehyde
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Lymphoma, Large B-Cell, Diffuse (genetics, pathology)
  • Male
  • Middle Aged
  • Paraffin Embedding
  • Tissue Banks
  • Transcriptome
  • Young Adult

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