Abstract |
Microthrombosis plays a key role in many cardiovascular diseases. Although it is not difficult to localize thrombus within large or middle-sized vessels, the noninvasive diagnostic regimen for the detection of microthrombus remains scarce. Here we developed a nanoagent by conjucting superparamagnetic iron-oxide nanoparticle with fluorophore and a targeting element, CREKA, a peptide with special affinity for fibrin. In a rat model of myocardial ischemia-reperfusion (MI/R), the multimodal nanoagents were readily and selectively accumulated within microthrombosis, which was detectable by both magnetic resonance and optical imaging modalities. The fibrin-targeted nanoagent could be expected to have utility not only in molecular imaging of fibrin, understanding the mechanisms of microcirculation disorders, but also in targeted therapy with fibrinolytic agents.
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Authors | Yanan Song, Zheyong Huang, Jianfeng Xu, Daoyuan Ren, Yu Wang, Xinde Zheng, Yunli Shen, Lili Wang, Hongxiang Gao, Jiayun Hou, Zhiqing Pang, Juying Qian, Junbo Ge |
Journal | Biomaterials
(Biomaterials)
Vol. 35
Issue 9
Pg. 2961-70
(Mar 2014)
ISSN: 1878-5905 [Electronic] Netherlands |
PMID | 24393265
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Ltd. All rights reserved. |
Chemical References |
- Dextrans
- Magnetite Nanoparticles
- Oligopeptides
- cysteinyl-arginyl-glutamyl-lysyl-alanyl
- ferumoxides
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Topics |
- Animals
- Blood Coagulation
(drug effects)
- Blood Platelets
(drug effects, pathology, ultrastructure)
- Dextrans
- Disease Models, Animal
- Humans
- Magnetite Nanoparticles
- Male
- Microscopy, Fluorescence
- Molecular Imaging
(methods)
- Multimodal Imaging
(methods)
- Myocardial Reperfusion Injury
(complications, diagnosis)
- Nanoparticles
(chemistry, ultrastructure)
- Oligopeptides
(pharmacology)
- Optical Imaging
- Particle Size
- Rats
- Rats, Sprague-Dawley
- Static Electricity
- Thrombosis
(complications, diagnosis)
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