Protective effect of tomatine against hydrogen peroxide-induced neurotoxicity in neuroblastoma (SH-SY5Y) cells.

Abstract

OBJECTIVES:

Reactive oxygen species can induce cell apoptosis, and oxidative stress has been implicated in a variety of neurodegenerative disorders. Tomatine, which is a naturally occurring steroidal glycoalkaloid isolated from Solanum cathayanum, has shown potent anti-oxidant properties.

METHODS:

In this study, we used the SH-SY5Y cell line as an in vitro model and investigated the protective effect of tomatine against hydrogen peroxide (H2 O2 )-induced neurotoxicity in SH-SY5Y cells.

KEY FINDINGS:

Tomatine might inhibit the release of cellular lactate dehydrogenase, increase anti-oxidant enzyme activity and glutathione content, reverse the downregulated protein expression of the brain-derived neurotrophic factor (BDNF), inhibit expression of Bax and activations of caspase-3 and caspase-9 in H2 O2 -induced SH-SY5Y cells.

CONCLUSIONS:

Tomatine exerted beneficially neuroprotective effect on H2 O2 -induced SH-SY5Y cells, mainly enhancing intracellular anti-oxidant enzyme activity and BDNF expression, inhibiting H2 O2 -induced oxidative stress as well as expression of Bax and activations of caspase-3 and caspase-9, alleviating H2 O2 -induced SH-SY5Y cell injury and cell death.

• Authors: Shao-lan Huang, Hai-bo He, Kun Zou, Cai-hong Bai, Yan-hong Xue, Jun-zhi Wang, Jian-feng Chen
• Journal: The Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 66 Issue 6 Pg. 844-54 (Jun 2014) ISSN: 2042-7158 [Electronic] England
• PMID: 24392651 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 Royal Pharmaceutical Society.

Chemical References

• Brain-Derived Neurotrophic Factor
• Neuroprotective Agents
• Tomatine
• Hydrogen Peroxide
• L-Lactate Dehydrogenase
• Caspase 3
• Caspase 9

Topics

• Brain-Derived Neurotrophic Factor (analysis)
• Caspase 3 (metabolism)
• Caspase 9 (metabolism)
• Cell Line, Tumor
• Humans
• Hydrogen Peroxide (toxicity)
• L-Lactate Dehydrogenase (metabolism)
• Neuroblastoma (pathology)
• Neuroprotective Agents (pharmacology)
• Tomatine (pharmacology)