This study aims to analyze the expression and clinical significance of
Filamin A (FLNA) in prostate
carcinoma and the
biological effect in its cell line by FLNA overexpression. Immunohistochemistry and Western blot were used to analyze FLNA
protein expression in 68 cases of
prostate cancer and 37 cases of normal tissues to study the influence of the upregulated expression of FLNA that might be found on PC-3 cell
biological effect. In the immunohistochemical analysis, the level of FLNA
protein expression was found to be significantly lower in
prostate cancer tissue than in normal tissues (P < 0.05). In the Western blot analysis, the relative amount of FLNA
protein in
prostate cancer tissue was found to be significantly lower than in normal tissues (P < 0.05). The level of FLNA
protein expression was not correlated with age and PSA concentration (P > 0.05), but it was correlated with T stages,
lymph node metastasis, clinic stage, and Gleason score (P < 0.05). The result of
biological function showed that PC-3 cell transfected FLNA had a lower survival fraction, a significant decrease in migration and invasion, and a lower matrix
metallopeptidase 9 (MMP-9)
protein expression compared with PC-3 cell untransfected FLNA (P < 0.05). FLNA expression decreased in
prostate cancer and correlated significantly with T stages,
lymph node metastasis, clinic stage, and Gleason score, suggesting that FLNA may play important roles as a negative regulator to
prostate cancer PC-3 cell by promoting the degradation of MMP-9.