Fabrication and characterization of a chemically oxidized-nanostructured porous silicon based biosensor implementing orienting protein A.

Abstract

Nanostructured porous silicon (PSi) elicits as a very attractive material for future biosensing systems due to its high surface area, biocompatibility and well-established fabrication methods. In order to engineer its performance as a biosensor transducer platform, the density of immunoglobulins properly immobilized and oriented onto the surface needs to be optimized. In this work we fabricated and characterized a novel biosensing system focusing on the improvement of the biofunctionalization cascade. The system consists on a chemically oxidized PSi platform derivatized with 3-aminopropyltriethoxysilane (APTS) that is coupled to Staphylococcus protein A (SpA). The chemical oxidation has previously demonstrated to enhance the biofunctionalization process and here "by implementing SpA" a molecularly oriented immunosensor is achieved. The biosensor system is characterized in terms of its chemical composition, wettability and optical reflectance. Finally, this system is successfully exploited to develop a biosensor for detecting asymmetric dimethylarginine (ADMA), an endogenous molecule involved in cardiovascular diseases. Therefore, this work is relevant from the point of view of design and optimization of the biomolecular immobilization cascade on PSi surfaces with the added value of contribution to the development of new assays for detecting ADMA with a view on prevention of cardiovascular diseases.

• Authors: Nelson Naveas, Jacobo Hernandez-Montelongo, Ruth Pulido, Vicente Torres-Costa, Raúl Villanueva-Guerrero, Josefa Predestinación García Ruiz, Miguel Manso-Silván
• Journal: Colloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 115 Pg. 310-6 (Mar 01 2014) ISSN: 1873-4367 [Electronic] Netherlands
• PMID: 24388860 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Elsevier B.V. All rights reserved.

Chemical References

• Biomarkers
• Immunoglobulins
• Staphylococcal Protein A
• dimethylarginine
• Water
• Arginine
• Silicon

Topics

• Arginine (analogs & derivatives, analysis)
• Biomarkers (analysis)
• Biosensing Techniques (methods)
• Immunoglobulins (metabolism)
• Microscopy, Fluorescence
• Nanostructures (chemistry, ultrastructure)
• Oxidation-Reduction
• Photoelectron Spectroscopy
• Porosity
• Silicon (chemistry)
• Spectroscopy, Fourier Transform Infrared
• Staphylococcal Protein A (metabolism)
• Water (chemistry)