Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus.

Abstract

Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.

• Authors: Chee-Keng Mok, Shih-Cheng Chang, Guang-Wu Chen, Yu-Lun Lo, Shu-Jen Chen, Ho-Sheng Wu, Ming-Tsan Liu, Feng-Yee Chang, Tzou-Yien Lin, Shin-Ru Shih
• Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (J Microbiol Immunol Infect) Vol. 48 Issue 4 Pg. 465-9 (Aug 2015) ISSN: 1995-9133 [Electronic] England
• PMID: 24388586 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Validation Study)
• Copyright: Copyright © 2013. Published by Elsevier B.V.

Chemical References

• Antiviral Agents
• RNA, Viral
• Viral Proteins
• Oseltamivir
• NA protein, influenza A virus
• Neuraminidase

Topics

• Antiviral Agents (pharmacology)
• Drug Resistance, Viral
• Humans
• Influenza A Virus, H7N9 Subtype (drug effects, enzymology, isolation & purification)
• Influenza, Human (virology)
• Molecular Sequence Data
• Mutation, Missense
• Neuraminidase (genetics)
• Oseltamivir (pharmacology)
• RNA, Viral (genetics)
• Reverse Transcriptase Polymerase Chain Reaction (methods)
• Sequence Analysis, DNA
• Taiwan
• Viral Proteins (genetics)