Association of duffy blood group gene polymorphisms with IL8 gene in chronic periodontitis.

Abstract

The antigens of the Duffy blood group system (DARC) act as a receptor for the interleukin IL-8. IL-8 plays an important role in the pathogenesis of chronic periodontitis due to its chemotactic properties on neutrophils. The aim of this study was to investigate a possible association of Duffy blood group gene polymorphisms with the -353T>A, -845T>C and -738T>A SNPs of the IL8 gene in chronic periodontitis. One hundred and twenty-four individuals with chronic periodontitis and 187 controls were enrolled. DNA was extracted using the salting-out method. The Duffy genotypes and IL8 gene promoter polymorphisms were investigated by PCR-RFLP. Statistical analyses were conducted using the Chi square test with Yates correction or Fisher's Exact Test, and the possibility of associations were evaluated by odds ratio with a 95% confidence interval. When analyzed separately, for the Duffy blood group system, differences in the genotype and allele frequencies were not observed between all the groups analyzed; and, in nonsmokers, the -845C allele (3.6% vs. 0.4%), -845TC genotype (7.3% vs. 0.7%) and the CTA haplotype (3.6% vs. 0.4%) were positively associated with chronic periodontitis. For the first time to our knowledge, the polymorphisms of erythroid DARC plus IL8 -353T>A SNPs were associated with chronic periodontitis in Brazilian individuals. In Afro-Brazilians patients, the FY*02N.01 with IL8 -353A SNP was associated with protection to chronic periodontitis.

• Authors: Emília Ângela Sippert, Cléverson de Oliveira e Silva, Jeane Eliete Laguila Visentainer, Ana Maria Sell
• Journal: PloS one (PLoS One) Vol. 8 Issue 12 Pg. e83286 ( 2013) ISSN: 1932-6203 [Electronic] United States
• PMID: 24386171 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CXCL8 protein, human
• Duffy Blood-Group System
• Interleukin-8

Topics

• Brazil
• Duffy Blood-Group System (genetics)
• Gene Frequency
• Genetic Association Studies
• Genotype
• Haplotypes
• Humans
• Interleukin-8 (genetics)
• Odds Ratio
• Periodontitis (genetics)
• Polymorphism, Genetic
• Promoter Regions, Genetic