5-Methoxytryptophan-dependent protection of cardiomyocytes from heart ischemia reperfusion injury.

Abstract

5-Methoxytryptophan (5-MTP), a catabolic product of tryptophan, can block Cox-2 overexpression in cancer cells as well as suppress cancer cell growth, migration and invasion. The aim of this study was to in vitro examine whether 5-MTP is able to reduce reactive oxygen species (ROS)-induced heart ischemia reperfusion injury and activate the cardiomyocyte's damage surveillance systems. Accordingly, rattus cardiomyocytes were treated with H2O2 as a heart ischemia reperfusion model prior to incubation with/without 5-MTP and proteomic analysis was performed to investigate the physiologic protection of 5-MTP in H2O2-induced ischemia reperfusion in cardiomyocyte. Our data demonstrated that 5-MTP treatment does protect cardiomyocyte in the ROS-induced ischemia reperfusion model. 5-MTP has also been shown to significantly facilitate cell migration and wound healing via cytoskeletal regulations. Additionally, two-dimensional differential gel electrophoresis (2D-DIGE) combined matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS) analysis showed that 5-MTP might modulate growth-associated proteins, cytoskeleton regulation, redox regulation and protein folding to stimulate wound healing as well as prevent these ischemia reperfusion-damaged cardiomyocytes from cell death through maintaining cellular redox-balance and reducing ER-stress. To our knowledge, we report for the first time the cell repair mechanism of 5-MTP against ischemia reperfusion-damage in cardiomyocytes based on cell biology and proteomic analysis.

• Authors: Hsiu-Chuan Chou, Hong-Lin Chan
• Journal: Archives of biochemistry and biophysics (Arch Biochem Biophys) Vol. 543 Pg. 15-22 (Feb 01 2014) ISSN: 1096-0384 [Electronic] United States
• PMID: 24384558 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• 5-methoxytryptophan
• Tryptophan
• Hydrogen Peroxide

Topics

• Animals
• Cell Death (drug effects)
• Cell Line
• Cell Survival (drug effects)
• Cytoprotection (drug effects)
• Endoplasmic Reticulum Stress (drug effects)
• Hydrogen Peroxide (pharmacology)
• Myocytes, Cardiac (drug effects, metabolism, pathology)
• Proteomics
• Rats
• Reperfusion Injury (genetics, metabolism, pathology, prevention & control)
• Transcriptome (drug effects)
• Tryptophan (analogs & derivatives, pharmacology)