Abstract |
Klebsiella 03 lipopolysaccharide (LPS) and Escherichia coli 09 LPS were previously shown to have potent adjuvant activities in augmenting antibody response and delayed-type hypersensitivity to protein antigens. The R form LPS extracted from the O-specific polysaccharide-less mutants derived from Klebsiella 03 and E. coli 09 strains were characterized by chemical and electrophoretic analyses. The adjuvant activities of the R-LPS in augmenting antibody response and DTH to protein antigens were much weaker than those of the parental LPS. The strength of the adjuvant activities of the R-LPS was similar to that of the activities of Salmonella minnesota LPS and Ra-LPS. By contrast, polyclonal B-cell activation effects of the R-LPS were stronger than those of the parental LPS. The common feature of the parental LPS is that their O-specific polysaccharides are mannose homopolymers ( mannans). From these results, it is suggested that mannose homopolymers as the O side-chains of LPS contribute to the action of LPS in enhancing strongly the T-cell dependent immune responses to protein antigens.
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Authors | M Ohta, N Kido, T Hasegawa, H Ito, Y Fujii, Y Arakawa, T Komatsu, N Kato |
Journal | Immunology
(Immunology)
Vol. 60
Issue 4
Pg. 503-7
(Apr 1987)
ISSN: 0019-2805 [Print] England |
PMID | 2438205
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Antibodies, Anti-Idiotypic
- Lipopolysaccharides
- Mannans
- Polysaccharides, Bacterial
- gamma-Globulins
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Topics |
- Adjuvants, Immunologic
(pharmacology)
- Animals
- Antibodies, Anti-Idiotypic
(biosynthesis)
- Chemical Phenomena
- Chemistry
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
(metabolism)
- Female
- Hypersensitivity, Delayed
- Klebsiella pneumoniae
(metabolism)
- Lipopolysaccharides
(immunology)
- Lymphocyte Activation
- Mannans
(immunology)
- Mice
- Mice, Inbred Strains
- Polysaccharides, Bacterial
(immunology)
- Salmonella
(metabolism)
- gamma-Globulins
(immunology)
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