[Effect of shuangshen ningxin formula on energy metabolism of myocardial ischemia/reperfusion rats].

Abstract

OBJECTIVE:

To investigate the effect of Shuangshen Ningxin (SSNX) formula on energy metabolism of myocardial ischemia/reperfusion rats.

METHOD:

The myocardial ischemia/reperfusion model of Wistar rats was established through the ligation of left anterior descending branch of coronary artery of for 40 min and the reperfusion for 2 h. The Wistar rats were randomly divided into six groups: the sham operation group, the model group, the Trimetazidine group (10 mg x kg(-1)) and SSNX groups (22.5, 45, 90 mg x kg(-1)). Preventive administration was conducted for 5 d. The operation was performed at 1 h on the day of the last administration. CK-MB assay kit was adopted to detect the activity of serum CK-MB. HPLC was used to determine ATP, ADP and AMP contents in myocardial tissues and calculate TAN and EC.

RESULT:

The preventive administration with SSNX could reduce the activity of serum CK-MB and increase ATP content and EC level in myocardial tissues (P < 0.01 or P < 0.05 vs. the model group).

CONCLUSION:

SSNX formula can maintain energy charge in cardiomyocytes and relieve ischemia/reperfusion injury by preserving ischemic myocardium ATP.

• Authors: Xue-Li Li, Jian-Xun Liu, Yu-Jie Guo
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 38 Issue 17 Pg. 2874-7 (Sep 2013) ISSN: 1001-5302 [Print] China
• PMID: 24380314 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drugs, Chinese Herbal
• shuangshen ningxin
• Adenosine Monophosphate
• Adenosine Diphosphate
• Creatine Kinase, MB Form

Topics

• Adenosine Diphosphate (metabolism)
• Adenosine Monophosphate (metabolism)
• Animals
• Creatine Kinase, MB Form (blood)
• Drugs, Chinese Herbal (administration & dosage)
• Energy Metabolism (drug effects)
• Humans
• Male
• Myocardial Ischemia (drug therapy, metabolism, surgery)
• Myocardial Reperfusion
• Rats
• Rats, Wistar