Altered peptidase activities in thyroid neoplasia and hyperplasia.

Abstract

BACKGROUND:

Papillary thyroid carcinoma (PTC), follicular thyroid adenoma (FTA), and thyroid nodular hyperplasia (TNH) are the most frequent diseases of the thyroid gland. Previous studies described the involvement of dipeptidyl-peptidase IV (DPPIV/CD26) in the development of thyroid neoplasia and proposed it as an additional tool in the diagnosis/prognosis of these diseases. However, very little is known about the involvement of other peptidases in neoplastic and hyperplastic processes of this gland.

METHODS:

The catalytic activity of 10 peptidases in a series of 30 PTC, 10 FTA, and 14 TNH was measured fluorimetrically in tumour and nontumour adjacent tissues.

RESULTS:

The activity of DPPIV/CD26 was markedly higher in PTC than in FTA, TNH, and nontumour tissues. Aspartyl aminopeptidase (AspAP), alanyl aminopeptidase (AlaAP), prolyl endopeptidase, pyroglutamyl peptidase I, and aminopeptidase B activities were significantly increased in thyroid neoplasms when compared to nontumour tissues. AspAP and AlaAP activities were also significantly higher in PTC than in FTA and TNH.

CONCLUSIONS:

These data suggest the involvement of DPPIV/CD26 and some cytosolic peptidases in the neoplastic development of PTC and FTA. Further studies will help to define the possible clinical usefulness of AlaAP and AspAP in the diagnosis/prognosis of thyroid neoplasms.

• Authors: Gorka Larrinaga, Lorena Blanco, Peio Errarte, Maider Beitia, Begoña Sanz, Itxaro Perez, Amaia Irazusta, Clara E Sánchez, Francisco Santaolalla, Leire Andrés, José I López
• Journal: Disease markers (Dis Markers) Vol. 35 Issue 6 Pg. 825-32 ( 2013) ISSN: 1875-8630 [Electronic] United States
• PMID: 24379520 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CD13 Antigens
• Glutamyl Aminopeptidase
• DPP4 protein, human
• Dipeptidyl Peptidase 4
• Pyroglutamyl-Peptidase I
• Serine Endopeptidases
• PREPL protein, human
• Prolyl Oligopeptidases

Topics

• Adenocarcinoma, Follicular (enzymology)
• Adult
• CD13 Antigens (metabolism)
• Carcinoma, Papillary (enzymology)
• Dipeptidyl Peptidase 4 (metabolism)
• Female
• Glutamyl Aminopeptidase (metabolism)
• Humans
• Hyperplasia (enzymology)
• Male
• Middle Aged
• Prolyl Oligopeptidases
• Pyroglutamyl-Peptidase I (metabolism)
• Serine Endopeptidases (metabolism)
• Thyroid Gland (enzymology, pathology)
• Thyroid Neoplasms (enzymology)