Antioxidant activity, total phenolic, total
flavonoid compounds and cytotoxicity to
cancer cell lines of
propolis extracts from two extraction methods were investigated in this study.
Propolis was collected from Phayao province and extracted with 70%
ethanol using maceration and sonication techniques. The
antioxidant activity was evaluated by DPPH assay. Total phenolic and
flavonoid compounds were also determined. Moreover, the cytotoxicity of
propolis was evaluated using MTT assay. The percentage
propolis yield after extraction using maceration (18.1%) was higher than using sonication (15.7%). Nevertheless,
antioxidant and
flavonoid compounds of the sonication
propolis extract were significant greater than using maceration.
Propolis extract from sonication showed
antioxidant activity by 3.30 ± 0.15 mg
gallic acid equivalents/g extract. Total phenolic compound was 18.3 ± 3.30 mg
gallic acid equivalents/g extract and
flavonoid compound was 20.49 ± 0.62 mg
quercetin/g extract. Additionally,
propolis extracts from two extraction methods demonstrated the inhibitory effect on proliferation of A549 and HeLa
cancer cell lines at 24, 48 and 72 hours in a dose-dependent manner. These results are of interest for the selection of the most appropriate method for preparation of
propolis extracts as potential
antioxidant and
anticancer agents.